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Addgene
Showing: 581 - 600 of 113262 results
  1. G6B-bio-His

    Plasmid
    #51644
    Purpose
    Expresses full-length Protein G6b precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    G6B (MPIG6B Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-SynaptoTAG2

    Plasmid
    #175275
    Purpose
    AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
    Depositor
    Insert
    EGFP-Synaptobrevin-2; tdTomato (Vamp2 )
    Use
    AAV
    Tags
    EGFP and tdTomato (P2A cleavage)
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pCiGSΔΨ

    Plasmid
    #108312
    Purpose
    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.
    Depositor
    Insert
    gag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    pCiGSΔΨ is a newer version of the pCiGS Gag expre…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. p2R3a-nosT

    Plasmid
    #71272
    Purpose
    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Insert
    nopaline synthase terminator
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid
    #98096
    Purpose
    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Insert
    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha A-crystallin 1 kb fragment (-1028/-1)
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. p2R3a-TagRFP-OcsT

    Plasmid
    #71270
    Purpose
    Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Insert
    TagRFP
    Use
    Gateway
    Tags
    4xGly linker and octaline synthase terminator
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. Danio alpha Ba-crystallin 3kb promoter/AcGFP

    Plasmid
    #98097
    Purpose
    aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Insert
    Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pLA2ScIfmTcIfm

    Plasmid
    #193792
    Purpose
    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Insert
    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLLacO-1 and PLTetO-1
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 581 - 600 of 113262 results