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pAAV-CAG-DIO-ChRmine-ST-P2A-H2B-mRuby3

Plasmid

#227779

Purpose

Cation channelrhodopsin ChRmine targeted to the neuronal soma and proximal dendrites in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

DIO-ChRmine-ST-P2A-H2B-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3

Plasmid

#240233

Purpose

Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

Myc-BirA*G3

Expression

Insect

Promoter

MT

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID

Plasmid

#240231

Purpose

Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

sfGFP-TurboID

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-sfGFP-TurboID-ER

Plasmid

#240232

Purpose

Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_BIP-sfGFP-TurboID-KDEL

Plasmid

#240223

Purpose

Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_spatzle-HA

Plasmid

#240227

Purpose

Gateway entry clone with spatzle tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

spatzle (spz Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-547 aa

Plasmid

#222657

Purpose

Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-547 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated histidine 548 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-409 aa

Plasmid

#222658

Purpose

Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-409 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated proline 410 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-244 aa

Plasmid

#222659

Purpose

Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-244 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated asparagine 245 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG-HA-Regnase-3 D252N

Plasmid

#222664

Purpose

Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

SFFV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 D252N-T2A-copGFP

Plasmid

#222661

Purpose

Lentiviral vector to express mouse Regnase-3 D252N

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

EF1α

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 WT-T2A-copGFP

Plasmid

#222660

Purpose

Lentiviral vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated stop codon prior to a T2A peptide

Promoter

EF1α

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 D252N

Plasmid

#222652

Purpose

For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 WT

Plasmid

#222651

Purpose

For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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