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pLenti-Zyxin-Full Length (aa1-564)-EGFP

Plasmid

#187692

Purpose

Visualization of Zyxin

Depositor

Mary Beckerle

Article

Hoffman et al Mol Biol Cell. 2012 May;23(10):1846-59.

Insert

Zyxin (Zyx Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Beta1 Q280* full-length

Plasmid

#221402

Purpose

Mammalian expression of human integrin beta1 Q280* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 K418* full-length

Plasmid

#221403

Purpose

Mammalian expression of human integrin beta1 K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N391* full-length

Plasmid

#221404

Purpose

Mammalian expression of human integrin beta1 N391* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Beta1 N562* full-length

Plasmid

#221405

Purpose

Mammalian expression of human integrin beta1 N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_DDEN29ENGD

Plasmid

#214320

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Mutation

DDEN29ENGD

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Full-length P229S talin1

Plasmid

#182993

Purpose

express FL variant P229S talin1 in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin1 P229S (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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full length png in pGEX4t

Plasmid

#112974

Purpose

For protein expression and purification of full-length Drosophila png

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

png (png Fly)

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Promoter

CMV

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE

Plasmid

#112872

Purpose

binary vector for ABE expression in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA cassette

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac1 human full-length CENP-E-mNeon-6xHis

Plasmid

#180484

Purpose

pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

Depositor

Julie Welburn

Article

Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

Insert

CENP-E (CENPE Human, human, Synthetic)

Tags

mNeon-6xHis

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pV238-vPE

Plasmid

#225258

Purpose

Mammalian expression of vPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

vPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-N22p-3XRFPnls

Plasmid

#75387

Purpose

N22p-3XRFPnls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

N22p

Use

Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

EFS

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pm-iATPSnFR1.1

Plasmid

#102549

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.1

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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