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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plenti-CAG-IKZF3-FLAG-IRES-GFP

Plasmid

#107388

Purpose

Lentiviral expression of IKZF3-FLAG

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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Bsrs103-RpaR-RpaI

Plasmid

#85159

Purpose

Constitutively expresses RpaR and inducibly expresses RpaI

Depositor

Jeff Hasty

Publication

Scott et al ACS Synth Biol. 2016 Sep 16;5(9):969-77.

Insert

RpaR, RpaI

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP41

Plasmid

#106407

Purpose

P-RAN-GFP41 (RANbody GFP41::vHRP)

Depositor

Joshua Sanes

Publication

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP41

Tags

HA and His

Expression

Mammalian

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR4_ELAVL1

Plasmid

#106103

Purpose

Entry vector for ELAVL1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Use

Gateway Cloning

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-NLS

Plasmid

#103840

Purpose

Flourescent marker for lacO arrays

Depositor

Karsten Rippe

Publication

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CFP-LacI-NLS

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

SV2

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-TagRFP-T

Plasmid

#103998

Purpose

Plasmid encoding TagRFP-T

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP-T

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2 mCherry Sec61B

Plasmid

#102246

Purpose

Expresses fusion of CRY2PHR with mCherry and Sec61B

Depositor

Bianxiao Cui

Publication

Che et al ACS Synth Biol. 2015 Oct 16;4(10):1124-3

Insert

CRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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p16 nlsGPS1-NR

Plasmid

#101082

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Publication

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

nlsGPS1-NR

Tags

cMyc

Expression

Plant

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

p16 (AT3G60245)

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCLIPf-NK1R Control Plasmid

Plasmid

#101125

Purpose

Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Neurokinin-1 Receptor, Truncated (TACR1 Human)

Tags

10xHis, CLIP-tag (CLIPf), and FLAG Epitope

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pAcGFP-C2-hSphK2

Plasmid

#84370

Purpose

Expression of AcGFP-SphK2 fusion protein in mammalian cells

Depositor

Anthony Don, Hugh Rosen

Publication

Don et al Biochem Biophys Res Commun. 2009 Feb 27;

Insert

SPHK2 (SPHK2 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only
pTRANS_253

Plasmid

#91125

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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NRCoC

Plasmid

#74300

Purpose

Mammalian expression of full-length treacle fused to GFP

Depositor

Rita Shiang

Publication

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MCS Lck only

Plasmid

#90411

Purpose

A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.

Depositor

Katharina Gaus

Publication

Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

Insert

Lck10+ Venus+mcherry

Expression

Mammalian

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pZS2oxySp-GFP-proD-oxyR

Plasmid

#78224

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTX171

Plasmid

#89258

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pTagBFP-Akt1

Plasmid

#86594

Purpose

Expresses TagBFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p-EGFP-C1-Flag-mFmr1(A)

Plasmid

#87913

Purpose

Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

Depositor

Stephanie Ceman

Publication

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499A

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 T56N

Plasmid

#86789

Purpose

mammalian expression of Rab12 T56N, constitutively GDP-bound mutant

Depositor

Peter McPherson

Publication

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Mutation

T56N, constitutively GDP-bound mutant

Promoter

CMV

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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