We narrowed to 8,601 results for: Set

1. pAbaMCi-GFP-gfp_pJMP2768

   Plasmid

   #208889

   Purpose

   Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette, sfGFP
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ChrII_ttTi5605_loxP_myo2_neoR

   Plasmid

   #194056

   Purpose

   Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ttTi5605
   

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-eSIBR

   Plasmid

   #71664

   Purpose

   EGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backbone

   Depositor

   Philip Washbourne

   Article

   Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

   Insert

   EGFP coding sequence followed by eSIBR cassette
   

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ChrV_oxTi365_loxP_myo2_neoR

   Plasmid

   #194057

   Purpose

   Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   oxTi365
   

   Expression

   Worm

   Promoter

   None

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.140

   Plasmid

   #184995

   Purpose

   Express Cas9 in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Promoter

   TetOn-3g

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSTK120-mCMV-TK

   Plasmid

   #22054

   Depositor

   Maria Castro

   Article

   King et al J Virol. 2008 May . 82(9):4680-4.

   Insert

   mCMV-TK-pA expression cassette
   

   Use

   Adenoviral

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pMyc-CMV1

   Plasmid

   #53582

   Purpose

   mammalian expression vector with Myc tag

   Depositor

   Linda Yu

   Article

   Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

   Type

   Empty backbone

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)

   Plasmid

   #167357

   Purpose

   Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t

   Depositor

   Michael Jensen

   Article

   Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

   Insert

   TDH3p:his6-mKate2-LipPks1-IDP1t
   

   Use

   Unspecified

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR_full-site

    Plasmid

    #186277

    Purpose

    Reporter plasmid for full-site selection assay

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pATF4-donor

    Plasmid

    #113805

    Purpose

    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP

    Depositor

    Kevin White

    Insert

    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    

    Use

    CRISPR

    Mutation

    rs140428747

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAP72_MS2

    Plasmid

    #220623

    Purpose

    Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Shuttle vector

    Expression

    Yeast

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-eSIBR

    Plasmid

    #71665

    Purpose

    intronically embedded eSIBR backbone in gateway middle entry vector (pDONR221)

    Depositor

    Philip Washbourne

    Article

    Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

    Insert

    intronic eSIBR cassette
    

    Use

    RNAi; Multi-site gateway

    Promoter

    None

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYR11 (XII-5:TDH3p:his6-mKate2-MBP-IDP1t)

    Plasmid

    #167356

    Purpose

    Integrative plasmid for cassette TDH3p:his6-mKate2-MBP-IDP1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    TDH3p:his6-mKate2-MBP-IDP1t
    

    Use

    Unspecified

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid

    #67279

    Purpose

    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    

    Tags

    linked to FlpO through an P2A ribosomal skipping …

    Expression

    Mammalian

    Promoter

    pdx

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. CasRX_Pac

    Plasmid

    #194803

    Purpose

    CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

    Depositor

    Alexander Paredez

    Article

    Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

    Insert

    CasRX
    

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTC201B

    Plasmid

    #52193

    Purpose

    for creating reversible targeting construct

    Depositor

    Tian Chi

    Article

    Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

    Insert

    promoter-trap cassette Neo-Ires-GFP
    

    Use

    Cre/Lox

    Tags

    Neo, GFP

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL70008

    Plasmid

    #50336

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRE57I-GNAc

    Plasmid

    #170637

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    Mini-Tn6677, GNAc cassette, lacI
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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