We narrowed to 146,921 results for: ins;

1. SOX9-T2A-H2B-EGFP repair template

   Plasmid

   #167972

   Purpose

   SOX9 reporter repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 9 (SOX9 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 3xFlag IFIT2

   Plasmid

   #53555

   Purpose

   mammalian expression of IFIT2

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT2 (IFIT2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX317-VRK1-K179E

   Plasmid

   #199644

   Purpose

   Expresses kinase-inactive VRK1 (K179E)

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   VRK1 (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K179E

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

   Plasmid

   #35512

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-His:CRY2:nls:VPR

   Plasmid

   #135989

   Purpose

   Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Inserts

   CRY2 domain fused to the VPR activation domain
   CRY2 domain fused to the VPR activation domain
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32a-hEPR

   Plasmid

   #199233

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proepiregulin preproprotein (EREG Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2133

   Plasmid

   #72249

   Purpose

   Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y450A, N497A, R661A, Q695A, and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mKate2-P2A-APEX2-2xFYVE_hrs

   Plasmid

   #67663

   Purpose

   Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-2xFYVE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_hSyn1-SIO-stGtACR1-FusionRed

    Plasmid

    #105678

    Purpose

    Expresses a soma-targeted GtACR1 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV5

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. BLADE-182

    Plasmid

    #134914

    Purpose

    sgRNA targeting GFP to be used in nanoblade system

    Depositor

    Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Insert

    GFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-LAMP1-mCherry-iLID

    Plasmid

    #174625

    Purpose

    Optogenetic coupling to lysosomes (LAMP1) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    LAMP1-mCherry-iLID (LAMP1 Human, Synthetic)
    

    Use

    Tags

    mCherry-iLID

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

    Plasmid

    #35511

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CaMKIIa-eArchT 3.0-EYFP

    Plasmid

    #35513

    Purpose

    Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArchT 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-spTorA-GFP-H6C

    Plasmid

    #168517

    Purpose

    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    spTorA-GFP-H6C
    

    Use

    Tags

    6xHisC tag and spTorA (signal peptide of E. coli …

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204188

    Purpose

    Doxycycline inducible lentiviral vector for human NOTUM overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NOTUM (NOTUM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPing

    Plasmid

    #47100

    Purpose

    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    Ping cDNA
    mPing
    hygromycin resistance gene
    

    Use

    Plant expression

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    CaMV35s and StUbi3

    Available Since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

    Plasmid

    #170177

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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