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pKSB-610 U6-sgRNA2 GGAA AGAG

Plasmid

#173672

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Publication

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM49981

Plasmid

#169087

Purpose

MoClo level 0 BvADHα coding sequence module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

BvADHα

Use

Synthetic Biology; Moclo cloning vector

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM921

Plasmid

#169086

Purpose

MoClo level 0 Db5GT coding sequence module

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Db5GT

Use

Synthetic Biology; Moclo cloning vector

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Publication

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDTN3997

Plasmid

#113337

Purpose

Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

Depositor

Suzanne Sandmeyer

Publication

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

multi-copy 2-pyrone synthase

Expression

Yeast

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pDM1210

Plasmid

#108987

Purpose

extrachromosomal expression vector, C-terminal mCherry tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

mCherry

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRVV669

Plasmid

#108283

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Publication

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgAY-CmR

Plasmid

#98938

Purpose

Conductive Curli Protein csgAY

Depositor

Urartu Seker

Publication

Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

Insert

csgAY

Tags

YYRKYKEYDDY

Expression

Bacterial

Promoter

tetO

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C3106

Plasmid

#91101

Purpose

Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Promoter

FMV 34S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Publication

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Expression

Bacterial

Promoter

LacUV5

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Publication

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Publication

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-PUb-SSA

Plasmid

#52910

Purpose

Ae aegypti PUb promoter Single Stranded Annealing reporter

Depositor

Zach Adelman

Publication

Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

Insert

Luciferase duplication-TALEN recognition sites

Use

Luciferase

Promoter

Ae aegypti polyubiquitin

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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p35ST

Plasmid

#51514

Purpose

pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule

Depositor

Daniel Voytas

Publication

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

T30 TALEN pair
us:NPTII repair template

Use

Plant expression t-dna

Tags

NLS and flag

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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Phi80 JQ929581

Plasmid

#38170

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Integration vector

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only
pRAJ12

Plasmid

#39246

Depositor

Alfonso Jaramillo

Publication

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ12m

Plasmid

#39247

Depositor

Alfonso Jaramillo

Publication

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ12 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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(pRZ219) AAV: U6-gRNA-EF1a-mScarlet

Plasmid

#254585

Purpose

AAV backbone expressing a U6-driven sgRNA with SapI spacer and mScarlet from the EF1a promoter

Depositor

Jesse Engreitz

Insert

sgRNA with SapI spacer

Use

AAV

Available since

May 13, 2026

Availability

Academic Institutions and Nonprofits only

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pLP857_α-syn:oScarlet KI donor

Plasmid

#239403

Purpose

AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

Depositor

Subhojit Roy

Publication

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

oScarlet

Use

AAV and CRISPR

Tags

oScarlet

Expression

Mammalian

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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