We narrowed to 10,611 results for: t7

1. pBH31(gfcD)

   Plasmid

   #176199

   Purpose

   Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Tags

   pelC leader-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBH31(gfcD-10xHis)

   Plasmid

   #176200

   Purpose

   Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

   Depositor

   Mark Saper

   Article

   Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

   Insert

   gfcD
   

   Tags

   pelC leader-10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET151-Vps4(101-437)

   Plasmid

   #87735

   Purpose

   Bacterial expression of N-terminally truncated S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1BR9-mCherrySTOP

   Plasmid

   #193866

   Purpose

   E. coli expression vector for N-ter GFP11 tagged mCherry.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   mCherry
   

   Tags

   6xHis and GFP11

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

   Plasmid

   #136546

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Tags

   ANXA2 (23-339) and His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-E2

   Plasmid

   #175242

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-APC11

   Plasmid

   #19898

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   APC11 (ANAPC11 Human)
   

   Tags

   HA and T7

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pET14_GFP5_Encap

   Plasmid

   #86408

   Purpose

   Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 5aa CLP tag
   Encapsulin
   

   Tags

   6x Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK357

   Plasmid

   #71568

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2236

    Plasmid

    #36687

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD4-TALEN_Left (Chd4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. CyPet-A206K-PRL2

    Plasmid

    #149685

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CyPet-A206K-PRL2
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    CyPet A206K

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6-rbNHERF1

    Plasmid

    #32942

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLC9A3R1 (SLC9A3R1 rabbit)
    

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

    Plasmid

    #168188

    Purpose

    HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b-At 14-3-3 omega

    Plasmid

    #44930

    Depositor

    Robert Ferl

    Article

    Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

    Insert

    14-3-3 omega (GRF2 Mustard Weed)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pTRCHisB-TRF2_M

    Plasmid

    #50490

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 438-500

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29b_Qcat9

    Plasmid

    #45137

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT9
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET29b_Qcat10

    Plasmid

    #45138

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT10
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTEV5_ RpMatB_4

    Plasmid

    #78208

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

    Insert

    matB (His6 C106A R286C T303A Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C T303A Q457C K488A

    Promoter

    T7

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTEV5_ RpMatB_3

    Plasmid

    #78207

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

    Insert

    matB (His6 C106A T167A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A T167A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET29b_Qcat3

    Plasmid

    #45131

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT3
    

    Tags

    5XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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