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NHR1-Flag pBacPAK8-3

Plasmid

#17323

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Expression

Mammalian

Mutation

G124I

Availability

Academic Institutions and Nonprofits only

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pPICZ:hTRAAK(W262S)

Plasmid

#130670

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, W262S

Availability

Academic Institutions and Nonprofits only

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Flag-SIRT1

Plasmid

#1791

Purpose

Mammalian expression of flag-tagged SIRT1

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-2xPH-TAPP1 WT

Plasmid

#161990

Purpose

Expresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PH-TAPP1 (PLEKHA1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pME-3x-FLAG-Ubiquitin (JDW 1098)

Plasmid

#224513

Purpose

A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3x-FLAG-Ubiquitin

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pYL192 (TRV1)

Plasmid

#148968

Purpose

Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

TRV RNA1

Use

Plant vector with trv rna1; this vector used with…

Mutation

P697S mutation in putative replicase. S239G and S…

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Article

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLViP-LifeAct-TagRFPT

Plasmid

#229700

Purpose

Lentiviral expression of LifeAct-TagRFPT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

TagRFPT

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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