We narrowed to 15,601 results for: LEA
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Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shNuf2
Plasmid#160961PurposeNuf2 shRNA in pMKO.1 retroviral vectorDepositorInsertNuf2 shRNA (Nuf2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(P1424G)
Plasmid#155128PurposeExpresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationP1424G; insert codes for EBA175 residues 1284 to …PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (H578A)
Plasmid#154858PurposeExpresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationHistidine 578 changed to AlaninePromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM4 (G470A/G471N)
Plasmid#154859PurposeExpresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM4 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationGlycine 470 changed to Alanine and Glycine 471 ch…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-PfROM1 (A144G/N145G)
Plasmid#154873PurposeExpresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1DepositorInsertPfROM1 (PF3D7_0506900 Synthetic)
UseTagstriple HAExpressionMammalianMutationAlanine 144 changed to Glycine and Asparagine 145…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(C104A/M247C)
Plasmid#155133PurposeExpresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
UseTagsHisExpressionBacterialMutationC104A/M247C, insert codes for GlpG residues 87-276PromoterT7Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM5
Plasmid#154166PurposeAedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM28
Plasmid#154165PurposeAedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virusDepositorInsertRel1A
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMtb MCM
Plasmid#138296PurposeExpression of Mtb MCM small and large subunitDepositorInsertsmutA
mutB
UseTagsHis tag and TEV cleavage siteExpressionBacterialMutationinserted TEV cleavage site between His-tag and m…PromoterT7Available sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts2
Plasmid#132714PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat NtsDepositorInsertshNts(2) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts1
Plasmid#132713PurposeEncodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts geneDepositorInsertshNts(1) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK353
Plasmid#129697PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120015170
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK355
Plasmid#129699PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminatorDepositorInsertsPrhaBAD
yfp
rhaS
ilvBN terminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD284
Plasmid#125073PurposeExpression of SO_4488 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD278
Plasmid#125070PurposeExpression of SO_2192 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationMutation in LacI S286L- Please see depositor comm…PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PD-L1-miSFIT-9-G, 15-C
Plasmid#124688PurposeTuning expression of PD-1DepositorInsertPD-L1 (CD274 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD2038
Plasmid#101378PurposeName: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.DepositorInsertBlue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
UseUnspecifiedTagsExpressionMutationBlue Heron Biotechnology Inc. bidirectional mutan…PromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -