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Addgene

We narrowed to 15,601 results for: LEA

Showing: 6101 - 6120 of 15601 results
  1. IBMc228

    Plasmid
    #161946
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR
    Depositor
    Insert
    pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid
    #154859
    Purpose
    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1
    Depositor
    Insert
    PfROM4 (PF3D7_0506900 Synthetic)
    Use
    Tags
    triple HA
    Expression
    Mammalian
    Mutation
    Glycine 470 changed to Alanine and Glycine 471 ch…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid
    #154873
    Purpose
    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1
    Depositor
    Insert
    PfROM1 (PF3D7_0506900 Synthetic)
    Use
    Tags
    triple HA
    Expression
    Mammalian
    Mutation
    Alanine 144 changed to Glycine and Asparagine 145…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pKM5

    Plasmid
    #154166
    Purpose
    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Insert
    Rel2 (long isoform)
    Use
    Tags
    FLAG
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pKM28

    Plasmid
    #154165
    Purpose
    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus
    Depositor
    Insert
    Rel1A
    Use
    Tags
    FLAG
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pMtb MCM

    Plasmid
    #138296
    Purpose
    Expression of Mtb MCM small and large subunit
    Depositor
    Inserts
    mutA
    mutB
    Use
    Tags
    His tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    inserted TEV cleavage site between His-tag and m…
    Promoter
    T7
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pCK353

    Plasmid
    #129697
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120015170
    Depositor
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120015170
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pCK355

    Plasmid
    #129699
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator
    Depositor
    Inserts
    PrhaBAD
    yfp
    rhaS
    ilvBN terminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pET-30a(+)-N-SH2

    Plasmid
    #111274
    Purpose
    express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Insert
    murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Use
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pDD2038

    Plasmid
    #101378
    Purpose
    Name: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.
    Depositor
    Insert
    Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Blue Heron Biotechnology Inc. bidirectional mutan…
    Promoter
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6101 - 6120 of 15601 results