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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

Plasmid

#62459

Purpose

expresses his-tagged C288S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 288 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-6

Plasmid

#172741

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV miRNA control plasmid

Plasmid

#31508

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Type

Empty backbone

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRiboI-Sec-APEX2m

Plasmid

#176845

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA p53, mitfa:Cas9

Plasmid

#118841

Purpose

Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

tp53 gRNA (tp53 Zebrafish)

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3C-BE3

Plasmid

#113412

Purpose

Expresses hA3C-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3C-BE3 (APOBEC3C S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3F-BE3

Plasmid

#113414

Purpose

Expresses hA3F-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3F-BE3 (APOBEC3F S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNAZeo(-)HSC73

Plasmid

#86030

Purpose

Expression of Heat shock chaperone 70 in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Heat-shock cognate protein 73 (HSPA8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-HBP1

Plasmid

#16178

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

HMG-box transcription factor 1 (HBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pLVXT-mWnt3

Plasmid

#121934

Purpose

doxycycline-inducible mouse Wnt3

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Wnt3 (Wnt3 Mouse)

Use

Lentiviral

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-CAG-EGFP

Plasmid

#153186

Purpose

CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-GFI1B

Plasmid

#16169

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Growth factor independent 1B (GFI1B Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
px330-UFM1 sgRNA2

Plasmid

#134634

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA2 (UFM1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA1

Plasmid

#134633

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA1 (UFM1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFRF1

Plasmid

#195801

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF1 (BFRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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G1BgSVNA-CDK4

Plasmid

#16573

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only