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E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

Plasmid

#171735

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

Depositor

Shin-ichi Arimura

Publication

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

Plasmid

#171727

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Publication

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

Plasmid

#171731

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Publication

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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E1_pENTR_L1-L4_NN_G1397-DddtoxA-C

Plasmid

#171730

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Publication

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST40-2XHA-Src-S4-S32-KD

Plasmid

#140298

Purpose

Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Publication

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

lacks the UD region

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-S32

Plasmid

#140303

Purpose

Expresses Flag-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Publication

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

lacks the KD region

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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UBC44

Plasmid

#25468

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubc44.001.167

Tags

His

Expression

Bacterial

Available since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-C9ORF80

Plasmid

#128418

Purpose

Express C9ORF80 in E. coli with a His tag (C-terminal)

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

C9ORF80 (INIP Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-mNRDc-E>A-V5

Plasmid

#51298

Purpose

expression of the inactive mutant of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Publication

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc E247A (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Mutation

E247A, enzymatic inactive mutant

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225062

Purpose

Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Publication

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

Plasmid

#225061

Purpose

Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Publication

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Publication

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB2H2*

Plasmid

#163834

Purpose

Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)

Depositor

Jerome Fox

Insert

SpecR

Use

Synthetic Biology

Expression

Bacterial

Mutation

TCPTP R222M

Promoter

Pro1;ProD;pLacZ-Opt

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KC

Plasmid

#134603

Purpose

Expresses IP3KC GFP tagged

Depositor

Christophe Erneux

Publication

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

IP3KC (ITPKC Human)

Tags

GFP

Expression

Mammalian

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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GUCY2C gRNA (BRDN0001144864)

Plasmid

#77563

Purpose

3rd generation lentiviral gRNA plasmid targeting human GUCY2C

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GUCY2C (GUCY2C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-338

Plasmid

#68795

Purpose

Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

Depositor

James Collins

Publication

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2

Tags

IRES, NLS, flag, and nls

Expression

Mammalian

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC28-Ub

Plasmid

#253392

Purpose

Expression in E. coli of ubiquitin.

Depositor

Kristijan Ramadan

Publication

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I44A

Plasmid

#253393

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44A mutation.

Depositor

Kristijan Ramadan

Publication

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44A

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I120A

Plasmid

#253394

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I120A mutation.

Depositor

Kristijan Ramadan

Publication

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I120A

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I44AI120A

Plasmid

#253395

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44AI120A double mutation.

Depositor

Kristijan Ramadan

Publication

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44AI120A

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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