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pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

Plasmid

#173231

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ThcCut1_pET29b

Plasmid

#215429

Purpose

Expression construct for ThcCut1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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c3GIC9

Plasmid

#62191

Purpose

col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

Depositor

Lukas Dow

Article

Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

Insert

humanized S. Pyogenes Cas9

Use

CRISPR

Tags

3x FLAG

Promoter

TRE3G

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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UMG-SP-3_pET29b

Plasmid

#215435

Purpose

Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-3

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NfPolyA_pET21b

Plasmid

#215413

Purpose

Expression construct for NfPolyA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NfPolyA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe

Plasmid

#183161

Purpose

Construct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry

Expression

Plant

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJB02

Plasmid

#111959

Purpose

Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

T7

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pM-mClover3

Plasmid

#228624

Purpose

Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mOtop1_pcDNA3

Plasmid

#114677

Purpose

This construct was used to express mouse Otop1 in HEK-293 cells

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 1 (Otop1 Mouse)

Expression

Mammalian

Mutation

SNP (rs49325632): Glycine 396 to Alanine

Promoter

CMV

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCD/NL-BH∆1

Plasmid

#41791

Purpose

Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

HIV-1 Gag/Pol and RRE sequences

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-PKCb1-mEGFP-N1

Plasmid

#112265

Purpose

ITRACKbeta/IDOCKSbeta Donor construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c beta (Prkcb Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCB19

Plasmid

#194757

Purpose

EGFP-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

EGFP-Tag

Mutation

none

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCB20

Plasmid

#194758

Purpose

ALFA-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

ALFA-Tag

Mutation

none

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

Plasmid

#227245

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMGS36 (GFP-ARF16-PB1)

Plasmid

#126581

Purpose

Construct to express GFP-ARF16-PB1 under the control of the CMV promoter

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

GFP

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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NylCA_pET28a

Plasmid

#215418

Purpose

Expression construct for NylCA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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