We narrowed to 15,723 results for: LEA

1. pCK355

   Plasmid

   #129699

   Purpose

   For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + ilvBN terminator

   Depositor

   John Heap

   Article

   Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

   Inserts

   PrhaBAD
   yfp
   rhaS
   ilvBN terminator
   

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKD284

   Plasmid

   #125073

   Purpose

   Expression of SO_4488 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_4488 (SO_4488 )
   lacI (lacI S286L- Please see depositor comments below)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD278

   Plasmid

   #125070

   Purpose

   Expression of SO_2192 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_2192 (SO_2192 )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Mutation in LacI S286L- Please see depositor comm…

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PD-L1-miSFIT-9-G, 15-C

   Plasmid

   #124688

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-L1 (CD274 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-30a(+)-N-SH2

   Plasmid

   #111274

   Purpose

   express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD2038

   Plasmid

   #101378

   Purpose

   Name: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
   

   Use

   Unspecified

   Mutation

   Blue Heron Biotechnology Inc. bidirectional mutan…

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN28

   Plasmid

   #100113

   Purpose

   expresses a chimeric cMYC-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN27

   Plasmid

   #100112

   Purpose

   expresses a chimeric cMYC-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCerulean-P2A-cMYC fused to a 'medium' VP16 domain (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN26

   Plasmid

   #100111

   Purpose

   expresses a chimeric cMYC-activator fusion

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCerulean-P2A-cMYC fused to a 'strong' VP16 domain (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMGF172

    Plasmid

    #96998

    Purpose

    ish1 mCherry insertion cassette with ura4+ gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + ura4(…

    Promoter

    N/A

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBD-Akt13C(WT)

    Plasmid

    #86576

    Purpose

    Expresses cleavable human full-length Akt1(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-AVITEV-GFP

    Plasmid

    #74052

    Purpose

    retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    AVITEV-GFP
    

    Use

    Retroviral

    Tags

    AVI-TEV-tag N-terminal to the GFP

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT TO myc Separase delta 55

    Plasmid

    #59824

    Purpose

    Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Cb2

    Plasmid

    #53529

    Purpose

    Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

    Depositor

    Gabor Igloi

    Article

    Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

    Insert

    mitochondrial arginyl-tRNA synthetase
    

    Tags

    His Tag and Thioredoxin

    Expression

    Bacterial

    Mutation

    changed Ser4 to Ala4 to permit NcoI cloning

    Promoter

    T7

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE224 human flag-Chibby-pCS3+FLT

    Plasmid

    #16824

    Depositor

    Randall Moon

    Article

    Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

    Insert

    flag-Chibby (CBY1 Human)
    

    Use

    Xenopus expression

    Tags

    flag

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

17. MDH1-miR-196a-2SM-PGK-GFP-Blast

    Plasmid

    #31512

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a2 (Mir196a-2 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

18. HOXB6-FLAGA-pSP65

    Plasmid

    #20994

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    In vitro trans/trans

    Tags

    FLAG

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA4-rB90

    Plasmid

    #18959

    Depositor

    Mariano Viapiano

    Article

    Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

    Insert

    Brevican C-terminus (Bcan Rat)
    

    Expression

    Mammalian

    Mutation

    Rat brevican C-terminus: Signal peptide aa 1-22 …

    Available Since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid

    #138212

    Purpose

    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub(T/A)-RLuc8N-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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