We narrowed to 7,105 results for: mme

1. pcDNA3-HA2-DDB1

   Plasmid

   #19909

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   Damage-specific DNA binding protein 1 (DDB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   please see depositor comment below

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xMx PylT_GFP 150TAG

   Plasmid

   #140016

   Purpose

   Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG, K46E (Please see depositor comments below)

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN565

   Plasmid

   #49990

   Purpose

   expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   T7 RNAP*
   

   Use

   Synthetic Biology

   Tags

   umuD degradation tag

   Expression

   Bacterial

   Mutation

   R632S

   Promoter

   pTac-symmetric

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-GGA1

   Plasmid

   #178459

   Purpose

   Expresses GGA1 in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

   Insert

   GGA1 (GGA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUW-tetO-Myb

   Plasmid

   #139826

   Purpose

   doxycycline-inducible expression of mouse Myb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Myb (Myb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P94H and Q619R- Please see depositor comment belo…

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCherry F8-2

   Plasmid

   #186034

   Purpose

   Express VHH F8-2 as a mCherry fusion in mammalian cells

   Depositor

   Isabelle Landrieu

   Article

   Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

   Insert

   VHH F8-2
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sumo3 PolQM1

   Plasmid

   #78462

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; Q2513R (please see depositor commen…

   Promoter

   T7

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pCerulean-VSVG

   Plasmid

   #11913

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. LifeAct-14 (LA-14)

   Plasmid

   #158750

   Purpose

   Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

   Insert

   LifeAct-14
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Deletion mutation for last three amino acids from…

   Promoter

   CMV immediate early

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-dDIO-vCre

    Plasmid

    #118029

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-dDIO-vCre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    See depositor comments below

    Promoter

    TRE

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 HisC EXO1b

    Plasmid

    #68268

    Purpose

    Mammalian expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pYPQ141-ZmUbi-RZ-As

    Plasmid

    #86196

    Purpose

    AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1 gRNA cloning site for ribozyme cleavage
    

    Use

    CRISPR

    Tags

    Hammerhead ribozyme and HDV ribozyme

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid

    #191164

    Purpose

    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (additional cutsites)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-EGFP-P2A-FusionRed

    Plasmid

    #191108

    Purpose

    Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    pAAV-CAG-EGFP-P2A-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

    Plasmid

    #100279

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Mutated catalytic domain (1025aa-End) (please see…

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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