We narrowed to 17,384 results for: puromycin
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Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…MutationSilent V205V mutation in myogenin gene to prevent…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only
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pAPM-D4 miR30-HDAC1 ts1
Plasmid#115852PurposeHDAC1 knockdownDepositorAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2601_eSpCas9-G2P
Plasmid#124203PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneExpressionMammalianAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-1
Plasmid#92156PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-1
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shID2 #1
Plasmid#83086PurposeLentiviral shRNA vector for inducible knockdown of human ID2DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1413 GFP-TDP43 VLIM-F
Plasmid#118802PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll VLIM to F in CTD of full-length TDP43Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX307 HRAS
Plasmid#117748PurposeOpen reading frame vector encoding HRASDepositorInsertRASH1 (HRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationClosed vector so will not read through the V5 reg…PromoterEF1aAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B CPM2
Plasmid#121163PurposeMammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)DepositorInsertZFP36L1 (TIS11B) charge pattern mutant 2
ExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TIS11B CPM2
Plasmid#121143PurposeMammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)DepositorInsertZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)
ExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MTOR HEAT
Plasmid#120709PurposeExpresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus productionDepositorInsertMTOR HEAT 1-9 (MTOR Human)
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 1-409PromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro
Plasmid#120564PurposeExpresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsHA-birA*ExpressionMammalianMutationQ61KPromoterCMVAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MPHOSPH8 ts2
Plasmid#115875PurposeMPHOSPH8 knockdownDepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-FAM208A ts1
Plasmid#115871PurposeFAM208a knockdownDepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-LIC-V5
Plasmid#120248PurposeFor LIC cloning with C-terminal V5 tagDepositorTypeEmpty backboneUseLentiviralTagsV5PromoterEF1aAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-AGM-VER9063 vpr
Plasmid#115825PurposeEncodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance markerDepositorInsertSIVAGM Vpr (Ver 9063)
UseLentiviralTags3x-FLAGPromoterSFFVAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I donor only control (F40-based tension sensor)
Plasmid#119188PurposeThe donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I acceptor only control (F40-based tension sensor)
Plasmid#119189PurposeThe acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR sgSHMT2_1
Plasmid#106308PurposeExpress Cas9 and sgRNA targeting SHMT2DepositorInsertsgRNA targeting SHMT2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-Myc-SNAP29
Plasmid#116944PurposeExpressing Myc-SNAP29 for mammalian cellsDepositorInsertsynaptosomal-associated protein, 29kDa (SNAP29 Human)
UseRetroviralTagsMycExpressionMammalianPromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only