We narrowed to 10,132 results for: yeast

1. pET26b_Nb.AD102

   Plasmid

   #131403

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody AD102
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS405 SEC66-VAC8-RFP

   Plasmid

   #166843

   Purpose

   Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Tags

   RFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFBP-4_1.sensor

   Plasmid

   #162844

   Purpose

   Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   4_1_RNA-device
   

   Expression

   Yeast

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBP-4_1m.mutant

   Plasmid

   #162869

   Purpose

   Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

   Depositor

   Matthias Heinemann

   Article

   Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

   Insert

   4_1m_RNA-device
   

   Expression

   Yeast

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJKW1813

   Plasmid

   #174003

   Purpose

   Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

   Depositor

   Jing-Ke Weng

   Article

   Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

   Inserts

   PpCYP90G4
   ATR1 (ATR1 Mustard Weed)
   

   Expression

   Yeast

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABF2-2EGFP-ter-URA3

   Plasmid

   #184768

   Purpose

   Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   ABF2 (ABF2 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS406-PHO5-GFP-CERT PH domain

   Plasmid

   #58725

   Purpose

   Expresses GFP-Tagged CERT PH domain in yeast

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
   

   Tags

   GFP and Myc

   Expression

   Yeast

   Promoter

   PHO5

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pGY416

   Plasmid

   #166660

   Purpose

   For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox

   Expression

   Yeast

   Mutation

   Cre: E340A, D341A

   Promoter

   GAL1

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGY466

   Plasmid

   #166661

   Purpose

   For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

   Depositor

   Gaël Yvert

   Article

   Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

   Insert

   LiCre
   

   Use

   Cre/Lox

   Expression

   Yeast

   Mutation

   Cre: E340A, D341A

   Promoter

   MET17

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDRF’- AmTryoshka1;3-GS

    Plasmid

    #100967

    Purpose

    Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-GS (AMT1;3 Mustard Weed)
    

    Expression

    Yeast

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Expression

    Yeast

    Promoter

    VTC4 (native promoter)

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p413TEF-CARKL

    Plasmid

    #51747

    Purpose

    overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

    Depositor

    Markus Ralser

    Article

    Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

    Insert

    SHPK (Shpk Mouse)
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMTKM02

    Plasmid

    #233474

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Nat selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMTKM04

    Plasmid

    #233476

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-G418 selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTKM06

    Plasmid

    #233478

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-Hyg selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMTKM08

    Plasmid

    #233480

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and split-URA selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS413-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221126

    Purpose

    FAP-tagged marker for the ER (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS415-TEF1pr-STE3-GFP

    Plasmid

    #221113

    Purpose

    GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    STE3-GFP
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS413-CUP1pr-NFAPoptim

    Plasmid

    #221110

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS413-CUP1pr-CFAPoptim

    Plasmid

    #221111

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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