Addgene

We narrowed to 1,071 results for: HST

Showing: 901 - 930 of 1071 results

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pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

Plasmid

#158952

Purpose

Express human RAD51(F129A) mutant in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

Insert

RAD51 (RAD51 Human)

Use

Tags

2X FLAG 2X STREP

Expression

Mammalian

Mutation

changed phenylalanine 129 to alanine

Promoter

CMV

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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YES1 Y537F

Plasmid

#51299

Purpose

Expression of YES1 Y537F

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y537F

Promoter

CMV

Available since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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TRAPPC8_pLX307

Plasmid

#98375

Purpose

Lentiviral expression of TRAPPC8

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TRAPPC8 (TRAPPC8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-HSF1Δ∼PRD

Plasmid

#71725

Purpose

plasmid expressing mic-his tag phosphorylation-deficient hHSF1

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

phosphorylation-deficient HSF1 (HSF1 Human)

Use

Tags

mac 6xHIS

Expression

Mammalian

Mutation

15 phosphorylation sites in RD mutated to Alanine

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK5

Plasmid

#53166

Purpose

Expresses GST-HA tagged human MEK5 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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hHSF1 K298R

Plasmid

#118362

Purpose

This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K298R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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shYES1 # 2

Plasmid

#42547

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
shYES1 # 1

Plasmid

#42546

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-RORb2

Plasmid

#73999

Purpose

Overexpression of RORb2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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YES1 (T348I) in pLX303

Plasmid

#42564

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T348I

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
TTR C10S S100C

Plasmid

#190010

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, S100C

Promoter

t7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-RORb2-IRES-GFPd2

Plasmid

#73998

Purpose

Plasmid expressing RORb2-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR1A dn-cHSF1

Plasmid

#134724

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

Expression

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

None

Available since

July 5, 2021

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK5

Plasmid

#53167

Purpose

Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF1616

Plasmid

#142811

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF519 (ZNF519 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1624

Plasmid

#144297

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF516 (ZNF516 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2001

Plasmid

#143993

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYEF2 (MYEF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2000

Plasmid

#143992

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYEF2 (MYEF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA2

Plasmid

#201629

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Use

Tags

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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hHSF1 S307A

Plasmid

#118364

Purpose

This plasmid expresses human HSF1 S307A mutant.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S307A

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-STING_gRNA3

Plasmid

#127640

Purpose

Knock-out of human STING

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2246

Plasmid

#141989

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCF12 (TCF12 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING

Plasmid

#102598

Purpose

Express STING for IFN-beta luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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