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pDonor-Prf1-mock-ires-mCherry

Plasmid

#209074

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-mock-ires-mCherry HDR template (parts of Prf1(S399Stop) with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroR

Plasmid

#190079

Purpose

express chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (TMPRSS2 Rhinolophus alcyone and Rhinolophus ferrumequinum, Synthetic)

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-HOXB4

Plasmid

#139829

Purpose

doxycycline-inducible expression of Human HOXB4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

HOXB4 (HOXA6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R249P- Please see depositor comment below

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-KLF4

Plasmid

#139828

Purpose

doxycycline-inducible expression of Human KLF4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S159AS197A

Plasmid

#131523

Purpose

Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Serine 159 to Alanine and Serine 197 to A…

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Hoxb6-3'UTR

Plasmid

#31523

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxb6-3'UTR (Hoxb6 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pLV.tetO.Nurr1

Plasmid

#234848

Purpose

3rd generation lentiviral vector, expresses Nurr1 under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Nurr1 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-FLAG-human cGAS-HA

Plasmid

#186849

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

FLAG and HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-human cGAS-HA

Plasmid

#186842

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTetOne-human cGAS-HA

Plasmid

#186896

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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HS69 pComb3xss anti-Homer1 nanobody

Plasmid

#134716

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE3G-human cGAS

Plasmid

#186853

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS without tags (Adamts1 Human)

Use

Retroviral

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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C1-82 pComb3xss anti-cFos nanobody

Plasmid

#145829

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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GC1 pComb3xss anti-Gephyrin nanobody

Plasmid

#134720

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC1 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pR26-CMVconst-mBMP7

Plasmid

#127375

Purpose

Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

BMP7 (Bmp7 Mouse)

Use

CRISPR and Mouse Targeting

Promoter

CMV enhancer/promoter

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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C1-62 pComb3xss anti-cFos nanobody

Plasmid

#145835

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-62 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCB306-GFP-mito

Plasmid

#229232

Purpose

Expresses GFP localized to mitochondria

Depositor

Suzanne Pfeffer

Article

Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

Insert

EGFP-MAOA (MAOA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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HS63 pComb3xss anti-Homer1 nanobody

Plasmid

#134715

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-BATF

Plasmid

#178451

Purpose

doxycycline-inducible expression of Human BATF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

BATF (BATF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
C2-77 pComb3xss anti-cFos nanobody

Plasmid

#145837

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-77 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
HS89 pComb3xss anti-Homer1 nanobody

Plasmid

#134717

Purpose

Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GC83 pComb3xss anti-Gephyrin nanobody

Plasmid

#134721

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S1-71 pComb3xss anti-cFos nanobody

Plasmid

#145832

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GC92 pComb3xss anti-Gephyrin nanobody

Plasmid

#145826

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GC92 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C2-66 pComb3xss anti-cFos nanobody

Plasmid

#145833

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-66 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C1-84 pComb3xss anti-cFos nanobody

Plasmid

#145830

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-84 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S2-77 pComb3xss anti-cFos nanobody

Plasmid

#145834

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S2-77 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C2-82 pComb3xss anti-cFos nanobody

Plasmid

#145838

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S1-54 pComb3xss anti-cFos nanobody

Plasmid

#145836

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody S1-54 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C2-58 pComb3xss anti-cFos nanobody

Plasmid

#145831

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-58 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GS8 pComb3xss anti-Gephyrin nanobody

Plasmid

#145827

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GS8 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS1 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145818

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS1 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SS41 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145823

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS41 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GS41 pComb3xss anti-Gephyrin nanobody

Plasmid

#145828

Purpose

Bacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-Gephyrin (Human) recombinant llama nanobody GS41 (GPHN L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NanoBRET PD-1/SHP2 Bidirectional Vector

Plasmid

#237016

Purpose

Express NanoLuc(R)-PD1/SHP2-HaloTag Fusion Proteins in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

PD-1 (PTPN11 Human)
SHP2 (PTPN11 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBC001 v3

Plasmid

#161711

Purpose

CMVp-EGFP-[barcode cloning site]-PGKp-Puro-WPRE

Depositor

Nozomu Yachie

Article

Nakayama et al Sci Immunol. 2024 May 24;9(95):eade3814.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV Promoter

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-mouse STING #1-gRNA-Cas9-Puro

Plasmid

#186892

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-chimpanzee cGAS-HA

Plasmid

#186873

Purpose

Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Chimpanzee cGAS with C-terminal HA tag (CGAS Pan troglodytes)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-mouse cGASdeltaN-HA

Plasmid

#186876

Purpose

Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Mouse cGAS truncation mutant (148-508 a.a.) with C-terminal HA tag (Cgas Mouse, Synthetic)

Use

Retroviral

Tags

HA

Mutation

N-terminal truncation

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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