We narrowed to 2,364 results for: EFS

1. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. myc-Miro1 (lacking TM)

   Plasmid

   #127613

   Purpose

   Human Miro1 with the transmembrane deleted

   Depositor

   Josef Kittler

   Article

   Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Deletion of transmembrane domain

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEXP5-NT-CuSeCat EE

   Plasmid

   #42569

   Depositor

   Ivan Korendovych

   Article

   Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

   Insert

   mutated calmodulin gene
   

   Use

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Valin…

   Promoter

   T7

   Available since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpCas9-NRCH-CGBE1

   Plasmid

   #198557

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-2TK-WW-CR-W3083F

   Plasmid

   #18076

   Depositor

   Marius Sudol

   Article

   Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

   Insert

   Dystrophin (DMD Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Second conserved Tryptophan (W) of the WW domain …

   Promoter

   Available since

   May 29, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pPKm-241

   Plasmid

   #104855

   Purpose

   pSIN-cytosFD-P2A-cytosFNR, expressing Synechococcus sp Fd and FNR, under EF-1alpha promoter

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   cytosFd-cytosFNR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. plex_307-EYFP-ccdB-g418

   Plasmid

   #201128

   Purpose

   pLEX_307 plasmid with N-terminal EYFP tag and g418 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. plex_307-mKate-ccdB-g418

   Plasmid

   #201125

   Purpose

   pLEX_307 plasmid with N-terminal mKate tag and g418 marker

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Tags

   mKate

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCas9-EGFP

   Plasmid

   #63592

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang, Phil Sharp

   Article

   Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid

    #198539

    Purpose

    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Sniper2L

    Plasmid

    #193856

    Purpose

    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSCV-F-del Casp9.IRES.GFP

    Plasmid

    #15567

    Depositor

    David Spencer

    Article

    Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

    Insert

    FKBP12(V36)-p30Caspase9 (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    FKBP12 and HA epitope

    Expression

    Mammalian

    Mutation

    FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Alexander Nectow, Eric Schmidt, Nathaniel Heintz

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. fluoPEER-GFP>BFP

    Plasmid

    #185477

    Purpose

    Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP-Y66-editing-site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. fluoPEER-HEK3_5bp-del

    Plasmid

    #185476

    Purpose

    Fluorescent reporter for a 5 basepair deletion at the HEK3 locus.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3 editing site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJET-CMV-GFP-P2A-K20-P2A-mCherry

    Plasmid

    #185618

    Purpose

    Ribosomal stalling reporter: positive control

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-K20(AAA)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJET-CMV-GFP-P2A-K0-P2A-mCherry

    Plasmid

    #185619

    Purpose

    Ribosomal stalling reporter: empty

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-empty-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJET-CMV-GFP-P2A-I15-P2A-mCherry

    Plasmid

    #185620

    Purpose

    Ribosomal stalling reporter: 15x isoleucine

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-I15-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pegRNA-GFP>BFP_Y66H-NGG

    Plasmid

    #185480

    Purpose

    pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP>BFP_Y66H-pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge