We narrowed to 4,253 results for: Gca

1. pU6-pegRNA-LRRK2-G2019S-3d

   Plasmid

   #180435

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV CAG Barcode5

   Plasmid

   #229067

   Purpose

   Expression mapping

   Depositor

   Scott Sternson

   Insert

   CAG Barcode5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV CAG Barcode3

   Plasmid

   #229064

   Purpose

   Expression mapping

   Depositor

   Scott Sternson

   Insert

   CAG Barcode3
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV CAG Barcode8

   Plasmid

   #229070

   Purpose

   Expression mapping

   Depositor

   Scott Sternson

   Insert

   CAG Barcode8
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. OgeuIscB AGC RNA

   Plasmid

   #222866

   Purpose

   This plasmid codes for the guide of the OgeuIscB with a AGC target

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB omega RNA with a (AGC)6 target sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Oct. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #222859

   Purpose

   This plasmid codes for the Slug-KH nickase with a sg(AGC)6

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   Slug-KHD10A+sgAGC
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, ITR2 and ITR2

   Expression

   Mammalian

   Mutation

   KH variant and D10A mutation

   Promoter

   H1 and eCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWT057c

   Plasmid

   #96870

   Purpose

   HHR-bsgRNA-FANCF

   Depositor

   David Liu

   Article

   Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

   Insert

   HHR-bsgRNA-FANCF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWT057d

   Plasmid

   #96871

   Purpose

   dHHR-bsgRNA-FANCF

   Depositor

   David Liu

   Article

   Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

   Insert

   dHHR-bsgRNA-FANCF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWT016h

   Plasmid

   #96854

   Purpose

   HHR-bsgRNA-GFP (17 nt blocker)

   Depositor

   David Liu

   Article

   Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

   Insert

   HHR-bsgRNA-GFP (17 nt blocker)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWT016d

    Plasmid

    #96855

    Purpose

    dHHR-bsgRNA-GFP (17 nt blocker)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    dHHR-bsgRNA-GFP (17 nt blocker)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWT049d

    Plasmid

    #96862

    Purpose

    guanine-agRNA (19 nt blocker with bulges, RFP activation)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    guanine-agRNA (19 nt blocker with bulges, RFP activation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWT049e

    Plasmid

    #96863

    Purpose

    (d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

    Depositor

    David Liu

    Article

    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

    Insert

    (d)guanine-agRNA (19 nt blocker with bulges, RFP activation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OA-1050G (white)

    Plasmid

    #132420

    Purpose

    express arrays of gRNA targeting White under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    white gRNA array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-3

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Use

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. L4440_BioBrick-hif-1-sgRNA-B

    Plasmid

    #177785

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hif-1 (hif-1 Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172670

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    SDHB pegRNA and SDHB_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. FgH1tUTG

    Plasmid

    #70183

    Purpose

    Inducible expression of guide RNA with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    H1-Tet-sgrna cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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