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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTK630

Plasmid

#114697

Purpose

Express RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA 5A-3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTK503

Plasmid

#114717

Purpose

Express mCherry-NuMA(WT) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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NK804

Plasmid

#166058

Purpose

Puromycin resistance and MRLC-mTFP expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

MRLC-mTFP

Use

S. rosetta expression

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-Vph1-HaloTag

Plasmid

#140149

Purpose

Plasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

VPH1

Expression

Yeast

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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NK802

Plasmid

#166056

Purpose

Puromycin resistance and mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

Pac puromycin resistance gene

Use

S. rosetta expression

Tags

mCherry

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pACS45

Plasmid

#204581

Purpose

GST-lamin A (389-646)

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

lamin A (389-646)

Tags

GST - lamin A (389-646)

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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YIplac211-Mup1-mScarlet2I

Plasmid

#140152

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

MUP1

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3

Plasmid

#203460

Purpose

jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

Gateway entry vector

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_pHoran4

Plasmid

#186975

Purpose

Expresses fluorescent pH sensor in mammalian cells

Depositor

Adam Cohen

Article

Hayward et al eLife. 2023 Oct 6;12:RP86833. doi: 10.75

Insert

pHoran4

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pN3-pHluorinCAAX

Plasmid

#123313

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CMV promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

pHluorinCAAX

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET28 His10-FcRɣ

Plasmid

#113021

Purpose

for purification of His10-FcRɣ intracellular domain

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

His10-FcRɣ intracellular domain

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182862

Purpose

Hetero-tetramer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mCherry2-mEGFP-mApple

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-Sec7-mScarlet2Ix6

Plasmid

#140151

Purpose

Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

SEC7

Expression

Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTK421

Plasmid

#114688

Purpose

RFP-Nano tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-HaloTagx2

Plasmid

#140150

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

GGA2

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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myr-palm-mApple

Plasmid

#182857

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mApple

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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glgP-gap1-

Plasmid

#101838

Purpose

deletes glgP_gap1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_244_245 operon)

Use

Cloning vector

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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