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Showing: 601 - 620 of 2070 results

  1. pCK341

    Plasmid
    #192640
    Purpose
    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dSpRY has s…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192640-plasmid-map-sequence-id-381518

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  3. pCK669

    Plasmid
    #192638
    Purpose
    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102
    Inserts
    Sp.pCas9-dxCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    SoxS has R93A and S101A mutations and dxCas9-NG h…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    192638-plasmid-map-sequence-id-381516

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  9. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

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  10. pECNUS2

    Plasmid
    #184885
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    184885-plasmid-map-sequence-id-435252

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  11. pECNUS3

    Plasmid
    #184886
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184886-plasmid-map-sequence-id-437894

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  12. pECNUS1

    Plasmid
    #184884
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184884-plasmid-map-sequence-id-437893

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  13. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  16. SpRYc

    Plasmid
    #175575
    Purpose
    Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
    Depositor
    Joseph Jacobson
    Article
    Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:
    Insert
    SpRYc
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    PID of SpRY grafted onto the N-terminal domain of…
    Promoter
    CMV
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    175575-plasmid-map-sequence-id-345065

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  18. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  19. pYPQ166-SpRY

    Plasmid
    #161520
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9
    Use
    CRISPR; Gateway compatible zsprycas9 entry clone
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161520-plasmid-map-sequence-id-325640

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  20. pYPQ262-ABE8e

    Plasmid
    #161523
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    ABE8e-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161523-plasmid-map-sequence-id-325555

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Showing: 601 - 620 of 2070 results