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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

Plasmid

#169188

Purpose

To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 D614G

Plasmid

#164437

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BQ.1.1 Spike

Plasmid

#193710

Purpose

Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BQ.1.1 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available since

Nov. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

Plasmid

#169187

Purpose

To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

Plasmid

#169186

Purpose

To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)

Use

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-SARS-CoV-2-Nucleocapsid

Plasmid

#204403

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-SARS-CoV-2-5'UTR

Plasmid

#184638

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

Plasmid

#107700

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3-P2A-CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin I

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b(+)_SARS-CoV-2_3CLpro-Q306A

Plasmid

#177334

Purpose

bacterial expression of active SARS-CoV-2 3C-like proteinase

Depositor

Christopher Overall

Article

Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Use

Tags

Factor Xa site-3xFlag-Myc-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available since

Nov. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 BA.2.75 Spike

Plasmid

#190012

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BA.2.75 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the BA.2 mutations (T19I, LPPA24S, G142D…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

Sept. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

Plasmid

#212487

Purpose

Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 D614G Spike

Plasmid

#185692

Purpose

Expresses codon-optimized full length SARS-CoV-2 D614G Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 D614G Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutation: D614G

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
YA1532: pAAV_ CAG-FLEX-paQuasAr3

Plasmid

#107702

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Plasmid

#178826

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP; HA

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2

Plasmid

#169463

Purpose

Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.1.7

Plasmid

#169459

Purpose

Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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