We narrowed to 10,402 results for: plasmids 101

1. pet30b-5M SrtA

   Plasmid

   #51140

   Purpose

   expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Article

   Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

   Insert

   S.aureus SrtA 5M
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-rev(CMV-TagBFP2)-EFS-mCherry-206-MRE

   Plasmid

   #235156

   Purpose

   Expresses the mCherry-based sensor for miR-206. TagBFP2 is expressed as a transduction reporter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   mCherry with a miR-206 MRE from Tpm4
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR_attB(bxb)_lox

   Plasmid

   #183762

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW-Cytosol-TurboID

   Plasmid

   #200961

   Purpose

   3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Cytosol-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUGW-Mito-TurboID

   Plasmid

   #200963

   Purpose

   3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   Mito-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUGW-ER-TurboID

   Plasmid

   #200965

   Purpose

   3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   ER-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAP2

    Plasmid

    #73211

    Purpose

    express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

    Depositor

    Luk Vandenberghe

    Article

    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

    Insert

    AAP2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiV_Neo_SIK3

    Plasmid

    #108103

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO7_Ef1a_SOX2

    Plasmid

    #18919

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pZac2.1-GfaABC1D-GAT3-HA-mCherry

    Plasmid

    #117722

    Purpose

    Expresses a GABA transporter GAT-3 (Slc6a11)

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Slc6a11 (Slc6a11 Mouse)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mammalian

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_rtTA-P2A-BFP_HygroR

    Plasmid

    #186738

    Purpose

    rtTA-P2A-BFP expression construct cloned into lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-S in pAR9 (rep/cap)

    Plasmid

    #174539

    Purpose

    encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

    Depositor

    Casey Maguire

    Article

    Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

    Insert

    VP1 of AAV9 containing the AAV-S 7-mer peptide insert
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

    Plasmid

    #232183

    Purpose

    Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6, hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUGW-Golgi-TurboID

    Plasmid

    #200964

    Purpose

    3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p15_attB(bxb)_lox

    Plasmid

    #183764

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PIV3HN-05 LC

    Plasmid

    #236190

    Purpose

    Produces the light chain of the human monoclonal antibody PIV3HN-05

    Depositor

    Jarrod Mousa

    Article

    Miller et al Nat Commun. 2024 Dec 30;15(1):10825. doi

    Insert

    PIV3HN-05 light chain
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PIV3HN-05 HC

    Plasmid

    #236189

    Purpose

    Produces the heavy chain of the human monoclonal antibody PIV3HN-05

    Depositor

    Jarrod Mousa

    Article

    Miller et al Nat Commun. 2024 Dec 30;15(1):10825. doi

    Insert

    PIV3HN-05 heavy chain
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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