12,913 results

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pACEBac1_Strep-Psc-BICD2(342-800)

Plasmid

#228832

Purpose

Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 342-800

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(711-800)_L742A

Plasmid

#228839

Purpose

Insect cell expression of the mutant C-terminus of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Mutation

aa 711-800, with L742A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_Nrg1-GV-2HA

Plasmid

#227104

Purpose

Nrg1 protein for luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

Nrg1-GV (Nrg1 Mouse)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I521A-peGFP-N1

Plasmid

#228577

Purpose

Expresses mouse TMEM16F-I521A-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I521A plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Tensin-2 Y483E

Plasmid

#201794

Purpose

Expresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

mutation Y483E

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Tensin-2 Y483F

Plasmid

#201795

Purpose

Expresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

mutation Y483F

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Tensin-2 Y483A

Plasmid

#201796

Purpose

Expresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Tensin-2 (Tns2 Mouse)

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

mutation Y483A

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-1 WT

Plasmid

#201798

Purpose

Expresses Talin-1 protein in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Talin-1 (Tln1 Mouse)

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoA gRNA #1

Plasmid

#198718

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoA gRNA #3

Plasmid

#198720

Purpose

Cas9-mediated knockout of RhoA in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoB gRNA #1

Plasmid

#198721

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoB gRNA #2

Plasmid

#198722

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #2

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoB gRNA #3

Plasmid

#198723

Purpose

Cas9-mediated knockout of RhoB in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoB (Rhob Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoC gRNA #1

Plasmid

#198724

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #1

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoC gRNA #2

Plasmid

#198725

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #2

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoC gRNA #3

Plasmid

#198726

Purpose

Cas9-mediated knockout of RhoC in mammalian cells #3

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoC (Rhoc Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-RhoA*

Plasmid

#198716

Purpose

Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

RhoA (Rhoa Mouse)

Expression

Mammalian

Mutation

It has mutations that is resistant to the RhoA gR…

Promoter

CAG

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

Plasmid

#127343

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Neo-TetO-Cdk13_Untagged

Plasmid

#127344

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

TetO Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-Neo-TetO-Cdk13_Nterm_FlagHa

Plasmid

#127345

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Piggybac transposase vector

Tags

Flag- HA- Tandem Epitopes

Expression

Mammalian

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

TetO Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk13_intron3_sg3_pX458

Plasmid

#127338

Purpose

sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk13_intron3_sg4_pX458

Plasmid

#127339

Purpose

sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 3 sgRNA (Cdk13 Mouse)

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk13_intron4_sg3_pX330

Plasmid

#127340

Purpose

sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk13_intron4_sg4_pX330

Plasmid

#127341

Purpose

sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 4 Targeting sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8/GW_TOPO_Cdk13_Untagged

Plasmid

#127342

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-GfaABC1D-eGFP-2A-mCherry_D4H

Plasmid

#194878

Purpose

Ratiometric D4H sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-GfaABC1D-eGFP-2A-mCherry_YDA

Plasmid

#194879

Purpose

Ratiometric YDA sensor for in vivo astrocyte-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-DiO-eGFP-2A-mCherry_YDA

Plasmid

#194881

Purpose

Ratiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-YDA

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ1022

Plasmid

#228762

Purpose

Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Pkd2 gRNAs (Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ1023

Plasmid

#228763

Purpose

Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

Depositor

Bradley Yoder

Insert

Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

human U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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