We narrowed to 15,723 results for: LEA

1. pFSW Doc2alpha D291N mutant

   Plasmid

   #128813

   Purpose

   To make virus expressing Doc2alpha D291 mutant

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Mutation

   D291N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSW Doc2beta D303N mutant

   Plasmid

   #128815

   Purpose

   To make virus expressing Doc2beta D303N mutant

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Mutation

   D303N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFSW Doc2alpha Halotag

   Plasmid

   #128817

   Purpose

   To make virus expressing Doc2alpha Halotag

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag-IRES-EGFP

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSW Doc2beta Halotag

   Plasmid

   #128818

   Purpose

   To make virus expressing Doc2beta Halotag

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag-IRES-EGFP

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PKAsub(T/A)-RLuc8N-NES

   Plasmid

   #138212

   Purpose

   Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub(T/A)-RLuc8N-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GRAB_g5-HT3.0

   Plasmid

   #208717

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Has Service

   AAV1, AAV2, and AAV8

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-XBP1 mNeonGreen NLS

   Plasmid

   #115968

   Purpose

   ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   X-box binding protein 1 (XBP1 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1a-Calr-KDEL-IRES-Puromycin

   Plasmid

   #134664

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   calreticulin (CALR Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 18-413

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-GRAB_g5-HT3.0

   Plasmid

   #208726

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZT-C13-R1

    Plasmid

    #62197

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Right CLYBL TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZT-C13-L1

    Plasmid

    #62196

    Purpose

    Chr.13/CLYBL TALEN expression vector

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Left CLYBL TALEN
    

    Use

    TALEN

    Promoter

    CMV

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-YE1-BE4max

    Plasmid

    #198546

    Purpose

    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-NG-ABE8e(V106W)

    Plasmid

    #198533

    Purpose

    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_RBMX-eA3A-UdgX-HF-nCas9

    Plasmid

    #163548

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-PGK-PYL1-sfGFP-Coilin

    Plasmid

    #122177

    Purpose

    expression of PYL1-SfGFP-Coilin, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    Coilin (COIL Human)
    

    Use

    Lentiviral

    Tags

    PYL1-SFGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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