We narrowed to 7,201 results for: mme

1. pCS2+ Gal4-GBP2-IRES-GBP7-p65

   Plasmid

   #50020

   Purpose

   Multi-purpose expression of a GFP-dependent transcription factor

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
   

   Use

   Multi-purpose expression

   Promoter

   CMV-immediate early promoter and SP6 promoter

   Available Since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLAREG

   Plasmid

   #73044

   Purpose

   Inducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.

   Depositor

   David McMillen

   Article

   Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-

   Inserts

   EGFP
   LacI
   

   Expression

   Yeast

   Promoter

   GAL1 promoter and hybrid dual-mode promoter-see c…

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b_EcSSB_1

   Plasmid

   #78203

   Purpose

   Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

   Depositor

   Martin Webb

   Article

   Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

   Insert

   ssb (G26C)
   

   Expression

   Bacterial

   Mutation

   G26C (please see depositor comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVenus-N-SLC35B2

   Plasmid

   #155038

   Purpose

   Transient expression of SLC35B2-Venus

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Cas13d-T2A-GFP(1-10)

   Plasmid

   #224566

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-GFP(1-10)
   

   Use

   CRISPR

   Tags

   T2A-split GFP(1-10)

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Cas13d-T2A-Citrine

   Plasmid

   #224565

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-Citrine
   

   Use

   CRISPR

   Tags

   T2A-Citrine

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL0-MtPT4Pro

   Plasmid

   #159414

   Purpose

   Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Insert

   MtPT4 Promoter
   

   Use

   Puc19-derived

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL0-MtBCP1Pro

    Plasmid

    #159415

    Purpose

    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtBCP1 Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-SENP8 (full length))

    Plasmid

    #16361

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP8 full length (SENP8 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    human SENP8 full length, see Depositor Comments

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b_EcSSB_2

    Plasmid

    #78204

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-

    Insert

    ssb (W88C)
    

    Expression

    Bacterial

    Mutation

    W88C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Expression

    Yeast

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid

    #73223

    Purpose

    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    dCas9 from S. pyogenes
    

    Tags

    c-myc

    Expression

    Bacterial

    Mutation

    Silent mutation at bp 1341 A->C to remove an E…

    Promoter

    PL22

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL1-MBP-4xDQNAT

    Plasmid

    #128390

    Purpose

    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    A302T- please see depositor comment

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKeratin-miRFP670nano

    Plasmid

    #127437

    Purpose

    Keratin labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    Keratin-miRFP670nano
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Keratin V282L - please see depositor comments bel…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(-)_mtZFN(-)2G

    Plasmid

    #62799

    Purpose

    mtZFN2G architecture bearing (-) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(-)2G
    

    Tags

    Internal FLAG tag immediately after F1B mitochond…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-)_mtZFN(+)2G

    Plasmid

    #62798

    Purpose

    mtZFN2G architecture bearing (+) FokI domain

    Depositor

    Michal Minczuk

    Article

    Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

    Insert

    mtZFN(+)2G
    

    Tags

    Internal HA tag immediately after F1B mitochondri…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6p-hDcp2

    Plasmid

    #72216

    Purpose

    bacterial expression of GST-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F146Y and E198K (please see depositor's comm…

    Promoter

    tac

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid

    #89869

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (please see depositor comments …

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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