We narrowed to 7,105 results for: mme

1. pFUW-tetO-NR4A3

   Plasmid

   #139818

   Purpose

   doxycycline-inducible expression of Human NR4A3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   NR4A3 (NR4A3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S240G- please see depositor comment below

   Promoter

   TRE-mCMV

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBTK569

   Plasmid

   #110614

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V32M (see comments below)

   Promoter

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMI91

   Plasmid

   #74888

   Purpose

   Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

   Depositor

   Piotr Marszalek

   Article

   Scholl et al Biomacromolecules. 2016 Jun 8.

   Insert

   Titin I27 x 9 (TTN Human)
   

   Use

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRB117.2

   Plasmid

   #181949

   Purpose

   aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial

   Mutation

   See Depositor Comments Below

   Promoter

   mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-EGFP-C1-HsNXF-1-5xmut_O

   Plasmid

   #147116

   Purpose

   Mammalian Expression of HsNxf-1-5xmut

   Depositor

   Elisa Izaurralde

   Article

   Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

   Insert

   HsNxf-1-5xmut (NXF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6M-Pak1 H83L H86L

   Plasmid

   #12211

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   PBD (p21-binding domain) mutant: H83L, H86L (see …

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN mCherry-TAX1BP1(V142S/ΔUBD)

   Plasmid

   #119690

   Purpose

   For mammalian expression of LIR mutant TAX1BP1 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V142S & UBD deletion (1-755), See depositor c…

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. sq5

   Plasmid

   #24499

   Depositor

   Bruce Conklin

   Article

   Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

   Insert

   Gs alpha (Gnas Rat)
   

   Use

   Tags

   HA (see comments)

   Expression

   Mammalian

   Mutation

   This is Gs alpha with the C-terminal amino acids …

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VVT1

    Plasmid

    #65779

    Purpose

    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SaCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJC6-GPT1-3xFLAG

    Plasmid

    #163448

    Purpose

    lentiviral overexpression vector for human GPT1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    GPT1 (GPT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SEP-Nanoluc-cyOFP1

    Plasmid

    #141082

    Purpose

    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of amino acids DISGG from positions 673 …

    Promoter

    EF1α

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Sumo3 PolQM1 deltains2

    Plasmid

    #78460

    Purpose

    Inducible expression in E. coli

    Depositor

    Sylvie Doublie, Susan Wallace

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; delta 2264-2315; Q2513R (see deposi…

    Promoter

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGL3-24p3/LCN2-282kB mut-LUC

    Plasmid

    #46867

    Purpose

    murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    NF- kB site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pGL3-24p3/LCN2-282C/EBP mut-LUC

    Plasmid

    #46868

    Purpose

    murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    24p3/lipocalin 2 proximal promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    CEBP site is mutated (see comments)

    Promoter

    24p3 promoter (inactive)

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

17. MSCV-Scara3-IRES-BFP

    Plasmid

    #117265

    Purpose

    Retroviral overexpression of Scara3

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Scara3 (Scara3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    LTR

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-At_PRORP1-His

    Plasmid

    #67869

    Purpose

    bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP1 (PRORP1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 36-572 (see comments)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. M72-IRES-tauRFP2-ACNF TV

    Plasmid

    #15512

    Depositor

    Peter Mombaerts

    Article

    Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

    Insert

    M72-IRES-tauRFP2-ACNF TV (Olfr160 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Targeting vector to insert IRES-tauRFP2-ACNF imme…

    Promoter

    Available since

    Aug. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-Ro2

    Plasmid

    #24503

    Depositor

    Bruce Conklin

    Article

    Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

    Insert

    RASSL Ro2 (OPRK1 Human)
    

    Use

    Tags

    FLAG and NLS from prolactin

    Expression

    Mammalian

    Mutation

    mutations in extracellular loops 2 (see comments …

    Promoter

    Available since

    June 16, 2010

    Availability

    Academic Institutions and Nonprofits only