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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA3-BRIPO

Plasmid

#215542

Purpose

BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Publication

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRIPO

Expression

Mammalian

Promoter

CMV promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDIV297

Plasmid

#204820

Purpose

AMA1 plasmid with amdS selection marker

Depositor

Uffe Mortensen

Publication

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

amdS

Use

Fungal expression

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans trpC promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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iCAP-BI-62-KanR

Plasmid

#203535

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI62 Cap

Use

AAV

Expression

Mammalian

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-35S_Long-6

Plasmid

#197698

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S promoter (1.3kb)

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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TH mAb_BCN53.1.1D10_LC_Mouse Ig Kappa

Plasmid

#182897

Purpose

Mammalian expression of TH mouse mAb LC gene

Depositor

Heng Zhu

Insert

anti-TH (human) light chain (TH Mouse)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

No

Promoter

CMV

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDN-LexAOx4-mNeonGreen

Plasmid

#192604

Purpose

Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

Depositor

Yingxiao Wang

Publication

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNeonGreen

Use

Unspecified

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pShoCAST-sgRNA_entry (BO1)

Plasmid

#181786

Purpose

Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAcCAST-sgRNA_entry (CJT83)

Plasmid

#181785

Purpose

Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Expression

Bacterial

Promoter

Lac and J23119

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Puro

Plasmid

#167871

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PibpA GFP pZa

Plasmid

#170076

Purpose

GFP expression under the control of E. coli ibpA promoter

Depositor

Urartu Seker

Publication

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

PibpA

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PclpB GFP pZa

Plasmid

#170074

Purpose

GFP expression under the control of E. coli clpB promoter

Depositor

Urartu Seker

Publication

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

PclpB

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7535

Plasmid

#169089

Purpose

MoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvCYP76AD1-Nos terminator

Use

Moclo cloning vector

Expression

Plant

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A

Plasmid

#129679

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Publication

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK206

Plasmid

#110588

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nanoluc

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTEI042

Plasmid

#105351

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Gal4UAS & 35S min promoter

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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sv272-HisMBP-TZAPZNF9-11

Plasmid

#87185

Purpose

bacterial expression of HisMBP-TZAPZNF9-11

Depositor

Eros Lazzerini Denchi

Publication

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Tags

HisMBP

Expression

Bacterial

Promoter

T7

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

Plasmid

#80911

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5G6G and PM,5G6G

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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