176,472 results
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Plasmid#122237PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.DepositorInsertsgRNA with cs2 at the 3' end of the constant region
UseCRISPR and LentiviralExpressionMammalianAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 N
Plasmid#149330PurposeGateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertN (N SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-sACE2(WT)-sfGFP
Plasmid#145171PurposeMammalian expression plasmid for soluble ACE2-super folder GFP fusionDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLAMP1-emiRFP670
Plasmid#136570PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertLAMP1-emiRFP670
TagsemiRFP670ExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-Cas9-gRNA-TagBFP2
Plasmid#124774Purpose3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-EFIRES-P-AtAFB2-mCherry
Plasmid#129716PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherryDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-FAH-rtTA3G
Plasmid#120309PurposeAn AAV vector that expresses rtTA3G under the c-fos promoterDepositorInsertrtTA3G
UseAAVPromoterc-fosAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Green Glifon50
Plasmid#126206Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon50
ExpressionMammalianAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX209-neo-active WRN
Plasmid#125788Purposeconstitutive, ectopic expression of human active WRNDepositorAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based NE sensor GRAB_NE1h
UseAAVPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertTurboID
UseDrosophila expressionAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP
Plasmid#111584PurposeAn AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.DepositorInsertmyrmScarlet-I-P2A-post-eGRASP
UseAAVPromotertetOAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralExpressionMammalianPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
Plasmid#103866PurposedPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-TFEBS211A-MYC (#805)
Plasmid#99957Purposemammalian expressionDepositorAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by