159,557 results

1. pDD426

   Plasmid

   #202079

   Purpose

   Frame +1/-0 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

   Plasmid

   #207805

   Purpose

   E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

   Depositor

   Koen Breyne

   Article

   Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

   Insert

   E-NoMi
   

   Use

   Lentiviral

   Tags

   3xFlag tag, copGFP, mCherry, and nanoluciferase

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG:H2B-EGFP

   Plasmid

   #32599

   Depositor

   Virginia Papaioannou, Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2004 Dec 24;4:33.

   Insert

   H2B-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TRUPATH Triple Gai1

   Plasmid

   #196048

   Purpose

   Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai1-RLuc8 (GNAI1 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Scarlet-Kif5a

   Plasmid

   #118745

   Purpose

   Expresses scarlet tagged Kif5a

   Depositor

   Beverly Koller

   Insert

   Kif5a cDNA (Kif5a Mouse)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE-30-HyPer3

   Plasmid

   #42132

   Purpose

   Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al ACS Chem Biol. 2012 Dec 20.

   Insert

   HYPER 3
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA4.TO-K5-2xCSTREP

   Plasmid

   #136154

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   K5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid

    #47549

    Purpose

    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    Empty sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR33.4r

    Plasmid

    #63198

    Purpose

    Expresses Cph8, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    Cph8
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.214_mCherry-PP7x12

    Plasmid

    #205556

    Purpose

    Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tdTomato-H2B-6

    Plasmid

    #58101

    Purpose

    Localization: Nucleus/Histones, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLCV2-APOBEC1-YTH

    Plasmid

    #178949

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTH-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDSG312

    Plasmid

    #115613

    Purpose

    surface-displayed Nb2, Arabinose-inducible; medium-copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plenti-FLAG-CAMK2D-ED

    Plasmid

    #221700

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K43R, D136N

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5FRT/Flag-LOC339287(MSL1)

    Plasmid

    #29762

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL1 (MSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

    Plasmid

    #89873

    Purpose

    expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    bArrestin2-TevC-P2A-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    R173H in TdTomato (Please see depositor comments …

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-EGFP

    Plasmid

    #89684

    Purpose

    pCAG promoter driving EGFP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge