We narrowed to 8,515 results for: Set

1. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

   Plasmid

   #178108

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA

   Plasmid

   #178109

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

   Plasmid

   #173209

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #173211

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

   Plasmid

   #173212

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TAC-OKMS-KRAB-dCas9

   Plasmid

   #120356

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-OK+9MS-KRAB-dCas9

   Plasmid

   #120357

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

   Plasmid

   #163927

   Purpose

   Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LMX1B-gRNA-C-del

   Plasmid

   #131516

   Purpose

   gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   LMX1B-gRNA-C-del
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

10. AcrIIC1-mCherry chimera #10

    Plasmid

    #128113

    Purpose

    Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrIIC1-mCherry chimera #10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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