12,458 results

1. pMIGII-TCR-B

   Plasmid

   #194388

   Purpose

   Retroviral expression of candidate orphan TCR

   Depositor

   Michael Carroll

   Article

   Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

   Insert

   TCR-B (Tcra, Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMIGII-TCR-C

   Plasmid

   #194389

   Purpose

   Retroviral expression of candidate orphan TCR

   Depositor

   Michael Carroll

   Article

   Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

   Insert

   TCR-C (Tcra, Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMIGII-TCR-D

   Plasmid

   #194390

   Purpose

   Retroviral expression of candidate orphan TCR

   Depositor

   Michael Carroll

   Article

   Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

   Insert

   TCR-D (Tcra, Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpyTag003(M115G)-Talin rod-mCherry

   Plasmid

   #210026

   Purpose

   Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 M115G mutation to modify SpyCatcher003 …

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpyTag003(M115A)-Talin rod-mCherry

   Plasmid

   #210025

   Purpose

   Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 M115A mutation to modify SpyCatcher003 …

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpyCatcher003(K31TAG)-Talin rod-mScarletI

   Plasmid

   #210022

   Purpose

   Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI
   

   Use

   Tags

   C-terminal mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpyTag003(V114T, V116T)-Talin rod-mCherry

   Plasmid

   #210027

   Purpose

   Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 V114T and V116T mutations to modify Spy…

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

   Plasmid

   #209089

   Purpose

   mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

   Plasmid

   #209090

   Purpose

   mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

   Insert

   PcdhgB2 (Pcdhgb2 Mouse)
   

   Use

   Tags

   PA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCX:γB2-EC5-VenusΔN3C9-P3

    Plasmid

    #209092

    Purpose

    mVenus-inserted PcdhgB2

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Combined γB2FL-FRET probe

    Plasmid

    #209094

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCX:γB2-EC1-G4S-mTQ2ΔC6

    Plasmid

    #209091

    Purpose

    mTurquoise2-inserted PcdhgB2

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Combined γB2ΔICD-FRET probe

    Plasmid

    #209093

    Purpose

    mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

    Depositor

    Takeharu Nagai

    Article

    Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid

    #210024

    Purpose

    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Encephalomyocarditis virus internal ribosome entr…

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-Talin head-SpyCatcher003(K31TAG)

    Plasmid

    #210023

    Purpose

    Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpyCatcher003 with K31TAG (Amber stop codon) muta…

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209061

    Purpose

    Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    KDM6A
    

    Use

    Gateway vector to be used for lr reaction

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLINE1ΔORF2-scFvFc

    Plasmid

    #206021

    Purpose

    The ORF2 sequence was removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLINE1ΔORF1-2-scFvFc

    Plasmid

    #206022

    Purpose

    The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLINE1mORF1-ΔORF2-scFvFc

    Plasmid

    #206023

    Purpose

    The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA TfR1-PuRRRE

    Plasmid

    #208327

    Purpose

    C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TfR1-PuRRRE
    

    Use

    Tags

    PuRRRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA TfR1-TREER

    Plasmid

    #208325

    Purpose

    C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TfR1-TREER
    

    Use

    Tags

    TREER

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA TfR1-TinyERRE

    Plasmid

    #208324

    Purpose

    C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TfR1-TinyERRE
    

    Use

    Tags

    TinyERRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmirGLO-3'UTR mouse Il13 mutDRACH

    Plasmid

    #207130

    Purpose

    Luciferase vector containing 3'UTR for mouse Il13 with mutated DRACH sites

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Interleukin 13 (Il13) 3'UTR (Il13 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    TGAGGAGAGACCATCCCTGGGCATCTCAGCTGTGGACTCATTTTCCTTT…

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCR 2.1-TOPO Fzd6 N352A HA

    Plasmid

    #208365

    Purpose

    Extracellular HA-tagged Fzd6 N352A (mouse)

    Depositor

    Danelle Devenport, Maureen Cetera

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Asparagine 352 to Alanine

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCR 2.1-TOPO Fzd6 S354I HA

    Plasmid

    #208364

    Purpose

    Extracellular HA-tagged Fzd6 S354I (mouse)

    Depositor

    Danelle Devenport, Maureen Cetera

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Serine 354 to Isoleucine

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR 2.1-TOPO Fzd6HA

    Plasmid

    #208363

    Purpose

    Extracellular HA-tagged Fzd6 (mouse)

    Depositor

    Danelle Devenport, Maureen Cetera

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid

    #209085

    Purpose

    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    TRE4G and hSyn

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

    Plasmid

    #207135

    Purpose

    DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSCALPS-FLAG-HA-mmuMettl3-LN

    Plasmid

    #207129

    Purpose

    Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Mettl3 (Mettl3 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-HA and Lambda Peptide (LN)

    Expression

    Mutation

    Promoter

    SSFV

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge