We narrowed to 15,724 results for: LEA

1. pCAG_iPE-C

   Plasmid

   #214735

   Purpose

   encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_iPE-C_bGH
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1a-GalT-IRES-Puromycin

   Plasmid

   #134862

   Purpose

   Expresses EGFP-tagged Golgi marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   beta 1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 83-398

   Promoter

   EF1a

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCVL.SSA TLR (Sce)

   Plasmid

   #45576

   Purpose

   Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with I-Sce I TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185bp, truncated 25 …

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

   Plasmid

   #176280

   Purpose

   Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mNaChBac-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpRY-ABE8e(V106W)

   Plasmid

   #198553

   Purpose

   Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CRISPRv2-sgNTC49-puro

   Plasmid

   #231989

   Purpose

   Knockout non-targeting control

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 and hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-DHFR F[3]-C (LEU2)

   Plasmid

   #177796

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[3]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-SpRY-NLS-6xHis (gbr2101)

   Plasmid

   #181743

   Purpose

   bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   T7

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. His-SUMO-Rad23A_pET11a

    Plasmid

    #169124

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hRad23A (RAD23A Human)
    

    Expression

    Bacterial

    Mutation

    wt

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUG-Pten

    Plasmid

    #140732

    Purpose

    Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-Pten (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    hUbiC

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. STAT3(Y705F)-TAL-Luc

    Plasmid

    #46933

    Purpose

    Luciferase Reporter for mutant STAT3

    Depositor

    Afshin Dowlati

    Article

    Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

    Insert

    STAT3(Y705F) (STAT3 Human)
    

    Use

    Luciferase

    Tags

    FLAG

    Mutation

    Tyrosine 705 to Phenylalanine

    Promoter

    TA Luciferase

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

13. alpha Synuclein-VC

    Plasmid

    #89471

    Purpose

    expresses human alpha Synuclein fused to the C-terminal part of Venus

    Depositor

    Bradley Hyman, Tiago Outeiro

    Article

    Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

    Insert

    SNCA (SNCA Human)
    

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 puro_shDDX58_230212

    Plasmid

    #167293

    Purpose

    Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    shRNA hsDDX58 (RIGI Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColdI-GST-mGTSF1

    Plasmid

    #214671

    Purpose

    Bacterial expression of GST-tagged mouse GTSF1

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF1 (Gtsf1 Mouse)
    

    Tags

    6x His and GST

    Expression

    Bacterial

    Promoter

    CspA

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP-SFFV-tagBFP-2A-ICP0-FxE

    Plasmid

    #235539

    Purpose

    To express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ICP0-FxE
    

    Use

    Lentiviral

    Mutation

    Deletion of the RING domain, ∆106-149.

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Delta(Pro-MP)ADAM10-HA

    Plasmid

    #65107

    Purpose

    Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM10 (ADAM10 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 19-455

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

19. SpCas9-ABE8e(V106W)

    Plasmid

    #198547

    Purpose

    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSynapsin_NLS_GFP_P2A_His6_NR1.

    Plasmid

    #170457

    Purpose

    Expresses histidine tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    Hexa-histidin

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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