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Hoxa4-3'UTR

Plasmid

#31524

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxa4-3'UTR (Hoxa4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pCVL.SSA TLR (Ani)

Plasmid

#45577

Purpose

Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

embedded I-Ani I TS from 163-185bp, truncated 25 …

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pClneoMyc MOB1 T35A

Plasmid

#47937

Purpose

expresses Myc tagged human MOB1 containing T35A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
MDH1-miR-324SM-PGK-GFP-Blast

Plasmid

#31518

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-324 (Mir324 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
3704 pGEX4T-3 MLL AD L2854A

Plasmid

#8876

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

L2854A: ILPSDIMDFVL --> ILPSDIMDFVA

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pFRT-TO-FLAG(N)-CDC73

Plasmid

#231751

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73deltaKIM

Plasmid

#231752

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(delta290-324)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-FLAG(N)-CDC73-YY/AA

Plasmid

#231753

Purpose

Expression in Flp-IN human cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Mammalian

Mutation

siCDC73#6 and #8 resistant; CDC73(Y290A, Y293A)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaNTD(700-1490)

Plasmid

#231734

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDK12 (CDK12 Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1490)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12-KiD(700-1082)

Plasmid

#231736

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDK12 (CDK12 Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(700-1082)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaRS(delta104-380)

Plasmid

#231737

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDK12 (CDK12 Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(delta104-380)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-CDK12RS(104-380)

Plasmid

#231738

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDK12 (CDK12 Human)

Use

Tags

Flag-GST-3C

Expression

Insect

Mutation

CDK12(104-380)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73deltaKIM(delta290-324)

Plasmid

#231739

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73-YY/AA(Y290A, Y293A)

Plasmid

#231740

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Martinez et al Molecular Cell. 01 Mar 2025. doi: 10.101

Insert

CDC73 (CDC73 Human)

Use

Tags

Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Promoter

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbATML1-2pro and NbATML1-1pro

Plasmid

#231154

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNAs targeting NbATML1-2pro and NbATML1-1pro

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase-P2A-H2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge