We narrowed to 14,023 results for: egf

1. pUC19-TgKI-MCS-eGFP-MCS-polyA

   Plasmid

   #174024

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2-UAS-zArchon1-KGC-EGFP-ER2

   Plasmid

   #108427

   Purpose

   Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zArchon1-KGC-EGFP-ER2
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   UAS

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

   Plasmid

   #223079

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

   Plasmid

   #194518

   Purpose

   ZSP promoter for transgenic enhancer assays in zebrafish

   Depositor

   Junsu Kang

   Article

   Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. WT1-2A-eGFP PGK PuroR Donor Plasmid

   Plasmid

   #82333

   Purpose

   WT1 targeting donor

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Inserts

   WT1 5' Arm (WT1 Human)
   WT1 3' Arm (WT1 )
   

   Use

   CRISPR and TALEN

   Tags

   2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRH-1365 LV EF1a-CD28 IRES-EGFP

   Plasmid

   #201921

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Enh eGFP Reporter-muKC2-X11

   Plasmid

   #59235

   Purpose

   Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

   Depositor

   Benjamin Yu

   Insert

   Krt7-ex2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Mu Hsp68 minimal promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. AAV8-hSyn-flex-miR30-eGFP-shDyn

   Plasmid

   #132716

   Purpose

   Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphin

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   GFP-shDyn (Pdyn Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin 1

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HIV7-Bcl2-CD28-CD3z-T2A-EGFR

    Plasmid

    #163465

    Purpose

    Lentiviral vector encoding a Bim-specific chimeric antigen receptor with a synthetic Bcl2 binding element

    Depositor

    David Baker

    Article

    Lajoie et al Science. 2020 Sep 25;369(6511):1637-1643

    Insert

    Bim-specific CAR
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

    Plasmid

    #32520

    Depositor

    Mustafa Sahin

    Article

    Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

    Insert

    Constitutively active Rheb (S16H) (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S16H to make constitutively active

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid

    #87803

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-EGFP barcode-1-SV40 polyA

    Plasmid

    #190865

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL

    Plasmid

    #218753

    Purpose

    Provides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

    Plasmid

    #191140

    Purpose

    Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-EGFP barcode-0-SV40 polyA

    Plasmid

    #190864

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)

    Plasmid

    #139990

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    VQR=D1135V/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-EGFP barcode-3-SV40 polyA

    Plasmid

    #190867

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-EGFP barcode-4-SV40 polyA

    Plasmid

    #190868

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode4
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Expression

    Mutation

    Promoter

    hUbC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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