We narrowed to 8,515 results for: Set

1. pFA0057

   Plasmid

   #131784

   Purpose

   Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer GFP cassette guide (gGFP) and 5' sgRNA
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9FnCpf1GG

   Plasmid

   #131013

   Purpose

   Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   GFP expression cassette
   direct repeat of FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. N229A Lo-MetQ

   Plasmid

   #118268

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Insert

   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x His Tag and Enterokinase Cleavage Site

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala)

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHyg-AID(31-114)

   Plasmid

   #99514

   Purpose

   Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(31-114) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   Expression

   Mutation

   internal inversion of nucleotides 265-312 of the …

   Promoter

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_B3200

   Plasmid

   #91079

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   none (begins with P2A to be fused to TALE-VPR_1)

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_B3400

   Plasmid

   #91080

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   none (begins with P2A to be fused to TALE-VP64_1)

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_A3101

   Plasmid

   #91048

   Purpose

   Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_A3301

   Plasmid

   #91049

   Purpose

   Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCT.VV.Orf199

   Plasmid

   #12887

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf199
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

10. DCT.VV.Orf200

    Plasmid

    #12888

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf200
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only