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We narrowed to 17,703 results for: puromycin

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Showing: 6261 - 6280 of 17703 results

  4. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Publication
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183081-plasmid-map-sequence-id-363415

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  5. pLP-PIGA2 (pLP300)

    Plasmid
    #168462
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Publication
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168462-plasmid-map-sequence-id-362578

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  7. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Publication
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

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  8. pLenti-CD74-ROS1 S1986F/G2032R/L2086F

    Plasmid
    #183822
    Purpose
    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 S1986F/G2032R/L2086F mutation in mammalian cells
    Depositor
    Aaron Hata
    Publication
    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899
    Inserts
    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of CD74 exon 1-6 and ROS1 exon…
    Expression
    Mammalian
    Mutation
    S1986F/G2032R/L2086F
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183822-plasmid-map-sequence-id-360503

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  9. pQCXIP-SHMY-PAUF

    Plasmid
    #182645
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence
    Depositor
    Martin Spiess
    Publication
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    Pancreatic adenocarcinoma upregulated factor (PAUF)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182645-plasmid-map-sequence-id-358618

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  13. pMP416

    Plasmid
    #134996
    Purpose
    dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Use
    Synthetic Biology
    Tags
    3xFLAG, E coli Dam(N132A), and dCas9
    Expression
    Mammalian
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134996-plasmid-map-sequence-id-267931

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  14. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179436
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Publication
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179436-plasmid-map-sequence-id-355459

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  15. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

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Showing: 6261 - 6280 of 17703 results