We narrowed to 16,540 results for: puromycin

1. pTRE3G-marmoset cGAS-HA

   Plasmid

   #186864

   Purpose

   Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Marmoset cGAS with C-terminal HA tag
   

   Use

   Retroviral

   Tags

   HA

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE3G-WHG cGAS-HA

   Plasmid

   #186867

   Purpose

   Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   White-handed gibbon cGAS with C-terminal HA tag
   

   Use

   Retroviral

   Tags

   HA

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57-CMV-MAAP-SL01

   Plasmid

   #218248

   Purpose

   1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-CMV-MAAP9

   Plasmid

   #218246

   Purpose

   1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIP-GFP-PKD C1ab

   Plasmid

   #221755

   Purpose

   Stable expression of GFP-PKD C1ab in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   Prkd1(138-343) (Prkd1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 138-343

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2 EGFP sgRNA4

   Plasmid

   #164934

   Purpose

   Expresses EGFP sgRNA4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCRISPRv2 NT sgRNA5

   Plasmid

   #164930

   Purpose

   Expresses Non-targeting sgRNA5 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA5 (verified for knockout)
   

   Use

   Lentiviral

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-EF1A-Puro-mCherry-RAB7-S72A

   Plasmid

   #221556

   Purpose

   Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Change serine 72 to alanine

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

   Plasmid

   #221552

   Purpose

   Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human Rab7A (RAB7A Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-IP-hWIPI2B-3×FLAG

    Plasmid

    #215510

    Purpose

    Expresses FLAG tagged human WIPI2B.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA068

    Plasmid

    #215994

    Purpose

    Fragmid fragment: (Selection) puromycin resistance

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; PuroR_v1.2
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. rSirt3 shRNA (pLKO.1 vector)

    Plasmid

    #216807

    Purpose

    shRNA against rat Sirt3

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

    Insert

    Sirt3 (Sirt3 Rat)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p667-UBC-MAFG-V5-miniTurbo

    Plasmid

    #207818

    Purpose

    C-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    MAFG-V5-miniTurbo
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Promoter

    UBC

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-Sik-YF

    Plasmid

    #210723

    Purpose

    Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

    Insert

    Protein tyrosine kinase 6 (Ptk6 Mouse)
    

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a-mXRCC1pd-eGFP

    Plasmid

    #206035

    Purpose

    Mammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    mXRCC1pd
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S105A, S186A, K188A, S195A, S221A, S222A,S238A, S…

    Promoter

    Ef1a

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-MAFG

    Plasmid

    #207817

    Purpose

    N-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-MAFG
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Promoter

    UBC

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1-ZNF746-C571A

    Plasmid

    #207826

    Purpose

    Dox-inducible expression of ZNF746-C571A

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-C571A (ZNF746 Human)
    

    Use

    Lentiviral

    Mutation

    C571A

    Promoter

    TRE

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXSIP-CMTM6-HA

    Plasmid

    #204710

    Purpose

    expresses CMTM6-HA in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    CMTM6 (CMTM6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPBpuro-ErbB1(F992)

    Plasmid

    #197365

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant (EGFR Human)
    

    Expression

    Mammalian

    Mutation

    Tyrosine 992 to Phenylalanine

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-mCherry-SPASTIN, L177D

    Plasmid

    #180649

    Purpose

    Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, L177D mutation, has siRNA resistan…

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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