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PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Promoter

Ef1a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRepair-mScI-CTNNB1

Plasmid

#153431

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mScarlet-i

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim1 (Bl/6)

Plasmid

#49205

Purpose

expresses Flag tagged mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (BL/6 allele) (Havcr1 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

Plasmid

#82701

Purpose

AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

Depositor

Jonathan Ploski

Article

de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

Insert

H1/TO gRNA Expression Cassette Containing Tet2 gRNA

Use

AAV and CRISPR

Tags

CMV promoter driving expression of TetR-P2A-GFP-K…

Expression

Mammalian

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi

Plasmid

#89740

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-mCherry-OptoJNKi

Plasmid

#89738

Purpose

Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-H2BmCherry-OptoJNKi

Plasmid

#89741

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Optogenetics

Tags

Histone2B and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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