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178,767 results

Showing: 6281 - 6300 of 178767 results

  1. pDONR223 SARS-CoV-2 N

    Plasmid
    #149330
    Purpose
    Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Publication
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149330-plasmid-map-sequence-id-296491

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  2. pcDNA3-sACE2(WT)-sfGFP

    Plasmid
    #145171
    Purpose
    Mammalian expression plasmid for soluble ACE2-super folder GFP fusion
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145171-plasmid-map-sequence-id-292613

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  4. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Publication
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113865-plasmid-map-sequence-id-220242

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  6. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid
    #129716
    Purpose
    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry
    Depositor
    Elina Ikonen
    Publication
    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    CRISPR and TALEN; Human safe harbor locus (a avs1…
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    EF1a
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129716-plasmid-map-sequence-id-254750

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  10. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118156-plasmid-map-sequence-id-239644

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  17. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Publication
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105842-plasmid-map-sequence-id-393690

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  20. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid
    #103866
    Purpose
    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103866-plasmid-map-sequence-id-199147

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Showing: 6281 - 6300 of 178767 results