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EF1as-ZIM3-Cas9-P2A-GFP

Plasmid

#239603

Purpose

Constitutive active CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-8

Plasmid

#237926

Purpose

Encodes TRBV7-8 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV7-8-TRAC (TRBV7-8 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-3

Plasmid

#237915

Purpose

Encodes TRBV6-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-3-TRAC (TRBV6-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV12-3

Plasmid

#237935

Purpose

Encodes TRBV12-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV12-3-TRAC (TRBV12-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV29-1

Plasmid

#237949

Purpose

Encodes TRBV29-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV29-1-TRAC (TRBV29-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV5-1

Plasmid

#237908

Purpose

Encodes TRBV5-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV5-1-TRAC (TRBV5-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV19

Plasmid

#237943

Purpose

Encodes TRBV19 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV19-TRAC (TRBV19 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV25-1

Plasmid

#237946

Purpose

Encodes TRBV25-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV25-1-TRAC (TRBV25-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-6

Plasmid

#237918

Purpose

Encodes TRBV6-6 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-6-TRAC (TRBV6-6 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-6

Plasmid

#237924

Purpose

Encodes TRBV7-6 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV7-6-TRAC (TRBV7-6 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV1-1

Plasmid

#237951

Purpose

Encodes TRAV1-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV1-1 (TRAV1-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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enDR3-TEV-mEGFP-NLS (pRS1451)

Plasmid

#240546

Purpose

Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.

Depositor

Roman Szczęsny

Article

Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

enDR3

Expression

Mammalian

Mutation

L52M

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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nb-enDR3-TEV-mEGFP-NLS (pRS1458)

Plasmid

#240547

Purpose

Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.

Depositor

Roman Szczęsny

Article

Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

nb-enDR3

Expression

Mammalian

Mutation

S48A, K50A

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C14A)-flag-tev-halo-his

Plasmid

#227159

Purpose

For T-REX experiments of CYP2A6 C14A mutant. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-halo-his

Plasmid

#227160

Purpose

For T-REX experiments of CYP2A6 C82A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-his

Plasmid

#227167

Purpose

For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C295A)-flag-tev-halo-his

Plasmid

#227156

Purpose

For T-REX experiments of cyp-33e1 C295A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1(C439A)-flag-tev-halo-his

Plasmid

#227157

Purpose

For T-REX experiments of cyp-33e1 C439A mutant. Less sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII-RFP-p2A-DAAO(R285A)-NES

Plasmid

#238919

Purpose

Mammalian expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in excitatory neurons

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO(R285A)-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKIIalfa

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO(R285A)-NES

Plasmid

#238921

Purpose

Expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO(R285A)-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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