We narrowed to 7,067 results for: FIE
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Plasmid#219948PurposeGolden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2DepositorInsertgRNA-MS2-3'end (S. pyogenes Cas9)
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2110-FKBP-GPA-20-114
Plasmid#222881PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc114
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2108-FRB-GPA-20-114
Plasmid#222879PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2109-FRB-GPA-20-11S
Plasmid#222880PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CG1358
Plasmid#218541PurposeDrosophila CG1358 cDNA (human codon optimized)DepositorAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro SPNS1_sg2
Plasmid#218531PurposesgRNA targeting human SPNS1DepositorInsertSPNS1 (SPNS1 Human)
UseCRISPR and LentiviralAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro SPNS1_sg1
Plasmid#218530PurposesgRNA targeting human SPNS1DepositorInsertSPNS1 (SPNS1 Human)
UseCRISPR and LentiviralAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v1 GFP FLVCR1_sg5
Plasmid#218521PurposesgRNA targeting human FLVCR1DepositorInsertFLVCR1 (FLVCR1 Human)
UseCRISPR and LentiviralAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CORO1B:1-489
Plasmid#210906PurposeInsect expression of full-length human CORO1B. N-terminal 6X His tag with TEV cleavage.DepositorInsertCORO1B:M1-A489 (CORO1B Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_AAMP:82-434
Plasmid#210904PurposeInsect expression of human AAMP fragment D82 to R434. N-terminal 6X His tag with TEV cleavage.DepositorInsertAAMP:D82-R434 (AAMP Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_RBBP7:1-411
Plasmid#210898PurposeInsect expression of human RBBP7 fragment M1 to E411. N-terminal 6X His tag with TEV cleavage.DepositorInsertRBBP7:M1-E411 (RBBP7 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmMRE11
Plasmid#208384PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine MRE11 gene.DepositorAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205098PurposeCre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogeneticsDepositorInsertmNeonGreen, eKL9h, and Volvox channelrhodopsin-1
UseAAVPromoterhSynAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pv9_MBD1_MBD-R22A-13XL-BASU
Plasmid#179417PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASUDepositorInsertMBD1.MBD-R22A-13XL-BASU
ExpressionMammalianPromoterCAGGSAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA
Plasmid#179402PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)DepositorInsertCBX7_Chromo - TAF3_Phd(DW890/891AA)
TagsAviTag and EGFPExpressionMammalianMutationPhd domain DW890/891AAPromoterCAGGSAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-td-sfGFP
Plasmid#180150Purposetd-sfGFP cre reporterDepositorInserttd-sfGFP
UseCre/LoxExpressionMammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-llo190-mc38minor
Plasmid#174604Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1DepositorInsertpalmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDM_N1_400
Plasmid#170474PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O-, O1, Lambda t1 in modified lacZ fragment
ExpressionBacterialMutationdiminished hairpins near O1 and diminished latent…PromoterT7A1Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only