We narrowed to 7,572 results for: Lif

1. pSIL-eGFP

   Plasmid

   #52675

   Purpose

   shRNA silencing plasmid with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Type

   Empty backbone

   Use

   RNAi

   Tags

   CMV-EGFP

   Expression

   Mutation

   Promoter

   CMV and U6

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTmpSm

   Plasmid

   #176228

   Purpose

   A template plasmid for amplification of the cI-hok-strA cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, strA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyLigase

   Plasmid

   #51722

   Purpose

   Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

   Depositor

   Mark Howarth

   Article

   Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

   Insert

   SpyLigase
   

   Use

   Tags

   His6 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEX128·gRNA

   Plasmid

   #187600

   Purpose

   Template for amplification of gRNA with Cas6 recognition site

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   gRNA template
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   no promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-hXRCC1

   Plasmid

   #52274

   Purpose

   Bacterial expression of human XRCC1 with N-terminal GST-tag

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL

   Plasmid

   #218750

   Purpose

   Provides NOT intersectional (Flp NOT Cre) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIM24 KanR

   Plasmid

   #67583

   Purpose

   Plant Gene Expression Vector

   Depositor

   Indu Maiti

   Article

   Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   M24

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTmpGm

   Plasmid

   #176229

   Purpose

   A template plasmid for amplification of the cI-hok-aacC4 cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, aacC4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSumo-His6-SUMO-AtTPR1(1-209)

   Plasmid

   #177858

   Purpose

   Bacterial Expression of TPR1

   Depositor

   Karsten Melcher

   Article

   Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

   Insert

   TPR1 (TPR1 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH_35S/mEGFP

    Plasmid

    #135321

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed hygromycin phosphotransferase selectable marker (hpt)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-CMV-N-Tandem-mCherry-EGFP

    Plasmid

    #123216

    Purpose

    N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-AS

    Plasmid

    #188870

    Purpose

    Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

    Depositor

    Fabio Pasin

    Article

    Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

    Insert

    none
    

    Use

    Synthetic Biology; Binary t-dna vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rac1

    Plasmid

    #55692

    Purpose

    Expression of mouse Rac1

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pENTR2b-mTurquoise2

    Plasmid

    #191014

    Purpose

    Gateway entry vector with the mTurquoise2 fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    mTurquoise2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. MRAS

    Plasmid

    #55661

    Purpose

    expression of mouse MRAS

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    MRAS (Mras Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pBS-67Sma

    Plasmid

    #49342

    Purpose

    Chlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region

    Depositor

    Anastasios Melis

    Article

    Chen et al Plant Biotechnol J. 2013 Sep;11(7):818-2

    Insert

    EcoRI 5.8 kB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSfinx-mYFP

    Plasmid

    #216206

    Purpose

    A simplified disease resistance assay using YFP-expressing Potato Virus X in N. benthamiana

    Depositor

    Takaki Maekawa

    Article

    Hasegawa et al Plant Physiol. 2024 Jul 8:kiae368. doi:

    Insert

    monomeric yellow fluorescent protein
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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