We narrowed to 8,324 results for: gnal

1. pMJ920

   Plasmid

   #42234

   Depositor

   Jennifer Doudna

   Article

   Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GFP, HA epitope, and NLS (nuclear localization si…

   Expression

   Mammalian

   Mutation

   codon-optimized synthetic DNA sequence

   Promoter

   CMV

   Available Since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWT35S-mCherry-NLS

   Plasmid

   #194049

   Purpose

   Transient expression of mCherry-NLS in plant cell (Nucleus)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

   Insert

   mCherry
   

   Use

   Tags

   SV40 (Nuclear localization signal)

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBI-MCS-EYFP

   Plasmid

   #58855

   Purpose

   The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJKR-O-mphR

   Plasmid

   #62570

   Purpose

   Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-RSV-tdTomato-2A-GCaMP6s

   Plasmid

   #80316

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Darrell Kotton

   Article

   Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

   Inserts

   tdTomato
   GCaMP6s
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   RSV

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

   Plasmid

   #211466

   Purpose

   Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   mito-mCherry-GFP1-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-RSV-tdTomato-2A-GCaMP6f

   Plasmid

   #80317

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Darrell Kotton

   Article

   Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

   Inserts

   tdTomato
   GCaMP6f
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   RSV

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. dTomato_LD 0_TEV

   Plasmid

   #58872

   Purpose

   MESA protease chain with dTomato ectodomain and no linker domain

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA protease chain with dTomato ectodomain and TEV protease
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid

    #99698

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV

    Depositor

    George Church

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-GFP1-10-HygR

    Plasmid

    #223675

    Purpose

    To check the split GFP signals in mitochondria (1)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GeNL(Ca2+)_480/pcDNA3

    Plasmid

    #85205

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_480
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAPV-DsRed2-ER-5

    Plasmid

    #170570

    Purpose

    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-PTS1-IRES-Puromycin

    Plasmid

    #134665

    Purpose

    Expresses EGFP-tagged peroxisome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    tripeptide SKL (Peroxisome targeting signal 1)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23-MYC

    Plasmid

    #86461

    Purpose

    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MYC

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ii-Str_SBP-EGFP-Ecadherin

    Plasmid

    #65288

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT-Bip-GFP:V5:KDEL

    Plasmid

    #69917

    Purpose

    An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

    Depositor

    Kenneth Irvine

    Article

    Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

    Insert

    GFP
    

    Use

    Tags

    Drosophila BiP signal sequence, KDEL ER targeting…

    Expression

    Insect

    Mutation

    Promoter

    pMT

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYHY3

    Plasmid

    #216109

    Purpose

    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT3630 signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    P1TDP-A21

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-3C-sGFP11_pcDNA3.1(-)

    Plasmid

    #182932

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3C-cleavable split GFP 11, FLAG tag, and HA signa…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-3C-sGFP1-10_pcDNA3.1(-)

    Plasmid

    #182931

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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