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pHAGE-PIK3CA-C420R

Plasmid

#116457

Purpose

Lentiviral expression of PIK3CA C420R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Tags

Expression

Mutation

C420R

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDK4-R24L

Plasmid

#116217

Purpose

Lentiviral expression of CDK4 R24L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK4 (CDK4 Human)

Use

Lentiviral

Tags

Expression

Mutation

R24L

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G469E

Plasmid

#116154

Purpose

Lentiviral expression of BRAF G469E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

G469E

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ESR1-L536Q

Plasmid

#116369

Purpose

Lentiviral expression of ESR1 L536Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

L536Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-nNOS/NOS1 [L121/133R]

Plasmid

#188170

Purpose

Mammalian Expression Plasmid of anti-nNOS/NOS1 (Human). Derived from hybridoma L121/133

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody (NOS1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAF1

Plasmid

#116785

Purpose

Lentiviral expression of RAF1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G466V

Plasmid

#116152

Purpose

Lentiviral expression of BRAF G466V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

G466V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-EAAC1 [N420/24R]

Plasmid

#206572

Purpose

Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N420/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-EAAC1 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc1a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-EAAC1 [N180/41R]

Plasmid

#206615

Purpose

Mammalian Expression Plasmid of anti-EAAC1 (Rat). Derived from hybridoma N180/41.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-EAAC1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc1a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G464V

Plasmid

#116148

Purpose

Lentiviral expression of BRAF G464V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

G464V

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 NSP2_nostop

Plasmid

#149305

Purpose

Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Anti-Miro2 [N384/63R]

Plasmid

#182114

Purpose

Mammalian Expression Plasmid of anti-Miro2 (Human). Derived from hybridoma N384/63.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Miro2 (Homo sapiens) recombinant mouse monoclonal antibody (RHOT2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd4

Plasmid

#102866

Purpose

Expresses mouse Fzd4 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 4 (Fzd4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T to C substitution at nucleotide 1149 of Fzd4 OR…

Promoter

EF1A

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

Plasmid

#208039

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa; 3MAS) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Expression

Mutation

3MAS mutant: K65R, K160R and K490R; mutated SIM

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF7B_nostop

Plasmid

#149325

Purpose

Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-ASNS-OE-IRES-Thy1.1

Plasmid

#192947

Purpose

Asparagine synthetase overexpression in murine cells

Depositor

Ping-Chih Ho

Article

Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

Insert

Asparagine synthetase (Asns Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Beta4-spectrin [N393/76R]

Plasmid

#177542

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G464E

Plasmid

#116146

Purpose

Lentiviral expression of BRAF G464E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

G464E

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-TrpV1 [N221/17R]

Plasmid

#177517

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EGFR-L861Q

Plasmid

#116272

Purpose

Lentiviral expression of EGFR L861Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

L861Q

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge