Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,549 results for: URE

Showing: 6341 - 6360 of 7549 results

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pETM10 with rat Δ192Annexin A11

Plasmid

#164498

Purpose

For bacterial expression of rat His-tagged Δ192Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ192Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat Δ188Annexin A11

Plasmid

#164497

Purpose

For bacterial expression of rat His-tagged Δ188Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat Δ188Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Mutation

N-terminal truncation

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD 5'UTR:YFP:Tnos (GB1483)

Plasmid

#160557

Purpose

Part 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

5'UTR:YFP:Tnos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirMecp2

Plasmid

#163704

Purpose

pAAV plasmid to induce expression of micro-RNA targeted against mouse Mecp2 and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirMecp2 and EGFP (Mecp2 Mouse, Aequorea victoria)

Use

AAV

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C128A

Plasmid

#162644

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C128A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C128 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C502A

Plasmid

#162645

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C502A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C502 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C544A

Plasmid

#162647

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C544A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C544 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 H581A

Plasmid

#162648

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 H581A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid H581 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32 C155A

Plasmid

#162649

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 C155A in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Mutation

Amino acid C155 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod(XL2V)-Doc

Plasmid

#157676

Purpose

Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc mutant XL2V with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-jph1a

Plasmid

#109551

Purpose

Multisite gateway vector for 3' tagging with junctophilin 1a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

junctophilin 1a (jph1a Zebrafish)

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab3ab

Plasmid

#109636

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab3ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3ab (rab3ab Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab35b

Plasmid

#109628

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab35b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab35b (rab35b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EHD1a-dEH

Plasmid

#109513

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD1a-delta-EH

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD1a (zgc:77049 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

dEH

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Delta-Hairpin

Plasmid

#136030

Purpose

EIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-1st-Hairpin

Plasmid

#136031

Purpose

EIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Disrupted-Hairpin

Plasmid

#136032

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Restored-Hairpin

Plasmid

#136033

Purpose

EIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-A:U-Hairpin

Plasmid

#136034

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Showing: 6341 - 6360 of 7549 results

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