We narrowed to 23,594 results for: promoter

1. vsv-CenpB 1-166-Sgo1 N61I-GFP

   Plasmid

   #108499

   Purpose

   expression of vsv-CenpB 1-166-Sgo1 N61I-EGFP (no PP2A binding)

   Depositor

   Susanne Lens

   Article

   Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

   Insert

   CenpB-Shugoshin 1 (SGO1 Human)
   

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CenpB 1-166 fusion before Sgo1, no PP2A binding N…

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR - Amplicon, JAK2 sgRNA 1

   Plasmid

   #70679

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pFIN-mIRBP156-CHER-RK-GFP-WPRE

   Plasmid

   #44593

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Inserts

   IRBP156-CHER
   RK-GFP
   

   Use

   Lentiviral

   Promoter

   human rhodopsin kinase (pos 3723-4019) and murine…

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWL1 (mANGPTL8-V5-His)

   Plasmid

   #109113

   Purpose

   Expresses V5 and His-tagged mouse ANGPTL8 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

   Insert

   ANGPTL8 (Angptl8 Mouse)
   

   Tags

   6x His and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMDC32-FIT2

   Plasmid

   #96990

   Purpose

   Express mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173205

   Purpose

   Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Dual U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-puro-mU6-CNPsgRNA

   Plasmid

   #126610

   Purpose

   Expresses CNP sgRNA under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   CNP sgRNA (Cnp Mouse)
   

   Use

   CRISPR

   Promoter

   mouse U6 promoter

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFAB3913

   Plasmid

   #47816

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 14 and BCD9
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDK4-luciferase active (880)

   Plasmid

   #86657

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   867 base pairs including up to coding nucleotide …

   Promoter

   CDK5

   Available Since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT-TO-vsv-Incenp wt-GFP

    Plasmid

    #108502

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124544

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55A, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124540

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124538

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-1 (GB1204)

    Plasmid

    #75405

    Purpose

    Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    AtU6-1 promoter
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid

    #70660

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

    Plasmid

    #61792

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhag 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Mutation

    miR-200c MRE-3 mutated

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pSLQ1371-sgITGB1-3

    Plasmid

    #121534

    Purpose

    sgITGB1-3 sequence: GTTCAGTGAATGGGAACAACG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB1-3
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Hmga2 ATG wt

    Plasmid

    #52034

    Purpose

    encodes wt HMGA2

    Depositor

    Julian Downward

    Article

    Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

    Insert

    Hmga2 (Hmga2 Mouse)
    

    Expression

    Mammalian

    Mutation

    starting ATG deleted

    Promoter

    CMV

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM549

    Plasmid

    #112531

    Purpose

    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Expression

    Bacterial

    Promoter

    J23107 and ProD

    Available Since

    Feb. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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