We narrowed to 6,524 results for: sas
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Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG_ATG101
Plasmid#223796PurposeExpression of recombinant protein for purification (co-expression with ATG13)DepositorInsertATG101 (ATG101 Human)
ExpressionMammalianAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-Ef1a-SOX2-3xFLAG
Plasmid#216197PurposeOverexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAsDepositorInsertSOX2 (SOX2 Human)
UseLentiviralAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LiOn*-CAG∞RFP
Plasmid#154019PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianMutationAll TTAA in vector backbone were replaced by sile…PromoterCAGAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞RFP
Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
ExpressionMammalianMutationH210PPromoterCAGAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
Plasmid#178528PurposeExpresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cellsDepositorInsertrtTA, SspB-mScarlet-I-MYPT (1-169aa)
ExpressionMammalianPromoterCAG, Tet responsive elementAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-NDP52
Plasmid#187828PurposePlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGenLenti V5-NIX
Plasmid#223731PurposeStable expression of protein in mammalian cell cultureDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGenLenti V5-BNIP3
Plasmid#223732PurposeStable expression of protein in mammalian cell cultureDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)
Plasmid#223770PurposeStable expression and inducible recruitment of protein in mammalian cell cultureDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-short_BrokenHeart (no BC)
Plasmid#86950PurposeAAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.DepositorInsertBrokenheart construct
UseAAVExpressionMammalianAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk12_Untagged
Plasmid#127178PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianPromoterTetO PromoterAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rBrokenheart
Plasmid#203394PurposeAAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.DepositorInsertBrokenheart
UseAAVExpressionMammalianPromoterEF1aAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
Tags6X Histidine Tag, TEV cleavage siteExpressionBacterialPromoterT7 lac promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-FLAG-METTL3-APPA
Plasmid#160253PurposeFor stable integration of catalytic inactive FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
TagsFLAGExpressionMammalianMutationchanges in aa 395–398, DPPW → APPAPromotertight TRE promoterAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
nes714tk/lacZ
Plasmid#47614Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZMutation714 bp from 3' end of 2nd intronPromoterHSV tk promoterAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-DEST-HOXA1
Plasmid#31209DepositorAvailable SinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
nes1852tk/lacZ
Plasmid#47613Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZMutation2nd intron onlyPromoterHSV tk promoterAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only