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pASZ-C-DM

Plasmid

#111235

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Publication

Grossmann et al Mol Syst Biol. 2015 Mar 26;11(3):794.

Insert

ccdB-cassette

Expression

Yeast

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pASZ-CN-DM

Plasmid

#111234

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Publication

Grossmann et al Mol Syst Biol. 2015 Mar 26;11(3):794.

Insert

ccdB-cassette

Expression

Yeast

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K127R)

Plasmid

#99543

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker

Depositor

Helle Ulrich

Publication

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRPR1_g2gRNA_RPR1t

Plasmid

#64388

Purpose

encodes g2 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g2 gRNA

Expression

Yeast

Promoter

pRPR1

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c2gRNA_RPR1t

Plasmid

#64380

Purpose

encodes c2 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c2 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_g1gRNA_RPR1t

Plasmid

#64387

Purpose

encodes g1 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g1 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c7gRNA_RPR1t

Plasmid

#64385

Purpose

encodes c7 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c7 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c1gRNA_RPR1t

Plasmid

#64379

Purpose

encodes c1 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c1 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c5gRNA_RPR1t

Plasmid

#64383

Purpose

encodes c5 gRNA

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c5 gRNA

Expression

Yeast

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pZHY566

Plasmid

#50608

Purpose

TALEN expression vector for yeast, N152,C18 architecture

Depositor

Daniel Voytas

Publication

Zhang et al Plant Physiol. 2012 Nov 2.

Type

Empty backbone

Use

TALEN

Expression

Yeast

Promoter

TEF

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pZHY565

Plasmid

#50607

Purpose

TALEN expression vector for yeast, N152,C18 architecture

Depositor

Daniel Voytas

Publication

Zhang et al Plant Physiol. 2012 Nov 2.

Type

Empty backbone

Use

TALEN

Expression

Yeast

Promoter

TEF

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pYDS2.0_AT118-H

Plasmid

#222846

Purpose

Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Publication

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-H

Expression

Yeast

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pTSK561

Plasmid

#190816

Purpose

For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

Depositor

Tatiana Karpova, Gunjan Mehta

Publication

Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

Insert

HaloTag

Use

Bacterial cloning vector

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNXgate33-3HA

Plasmid

#105075

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pML104-KanMX-sgRNAv2

Plasmid

#254712

Purpose

Yeast CRISPR-Cas9 backbone with sgRNA cassette for rapid PCR-based guide insertion; KanMX/G418 selection in S. cerevisiae, Amp/Kan propagation in E. coli.

Depositor

Brian Strahl

Publication

Rostamian et al STAR Protoc. 2026 Jul 28;7(3):104751. do

Insert

YKU70 sgRNA (YKU70 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52 promoter

Available since

Aug. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pRS415-Cas9-VPR

Plasmid

#163971

Purpose

Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

Depositor

Jiazhang Lian

Publication

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Insert

Cas9-VPR

Use

Synthetic Biology

Expression

Yeast

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB1975 (pUC19-PTEF1-PPGK1)

Plasmid

#73296

Purpose

Example plasmid containing a bidirectional promoter

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

pTEF1-pPGK1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PMV-ycrtI

Plasmid

#65342

Purpose

systhesis of crtI gene in the beta-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtI

Use

Synthetic Biology

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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PMV-ycrtYB

Plasmid

#65343

Purpose

systhesis of crtYB gene in the beta-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtYB

Use

Synthetic Biology

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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