164,884 results

1. pUFlip-floxed2A-mRFP-; HE1A:BFP -2

   Plasmid

   #180009

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSW036

   Plasmid

   #140034

   Purpose

   KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Yellow fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cpt

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_5x Gal4 UAS

   Plasmid

   #79119

   Purpose

   Base vector for cloning Gal4DBDVP64 inducible genes

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18T-mini-Tn7T-Gm

   Plasmid

   #63121

   Purpose

   mini-Tn7 vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 17, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pX330A-1x2

   Plasmid

   #58766

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

   Plasmid

   #170378

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin promoter

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER13 miR-200c-141

   Plasmid

   #81020

   Purpose

   Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

   Depositor

   Thomas Westbrook, Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   miR-200c (MIR200C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-GGamma10-GFP2

   Plasmid

   #166779

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma10-GFP2 (GNG10 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-human Igf2bp2-IRES-GFP

   Plasmid

   #91890

   Purpose

   Retroviral expression of human Igfbp2

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Igf2bp2 (IGF2BP2 Human)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3311

    Plasmid

    #144787

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB3 (CREB3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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