We narrowed to 14,579 results for: pET

1. pET15b+PCNA2 E105C/L171C

   Plasmid

   #66178

   Purpose

   Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C, L171C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-22HT-MSI1(7-192)

   Plasmid

   #60245

   Purpose

   Express the RBD of mouse MSI1 in bacterial cells for protein purification

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   contains aa7-192 of Msi1

   Promoter

   T7

   Available since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. M26L DJ-1 TEV site pET15b

   Plasmid

   #60686

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26L DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26L and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET-DEST42-ARL4C-V5-His6

   Plasmid

   #67423

   Purpose

   Expression of hArl4c with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL7 (ARL4C Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-DEST42-ARL4-V5-His6

   Plasmid

   #67419

   Purpose

   Expression of hArl4 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4 (ARL4A Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 105 A to T; silent mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET15b/EYFP-GgVcl 1-258

   Plasmid

   #46263

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

   Insert

   Vcl 1-258 (VCL Chicken)
   

   Use

   Tags

   EYFP and His

   Expression

   Bacterial

   Mutation

   aa 1-258 (VD1)

   Promoter

   T7

   Available since

   July 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet 3d double tentacle delete CP

   Plasmid

   #13301

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Capping protein alpha 1 (with 28 amino acids dele…

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET-21a(+) SurE D9A

   Plasmid

   #12686

   Depositor

   SeWon Suh

   Article

   Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

   Insert

   SurE
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Aspartate 9 to Alanine

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

9. HOXA9 (murine) no-tags pET

   Plasmid

   #8493

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   makes protein by Western

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET22b-EC12-129/138

    Plasmid

    #47926

    Purpose

    Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    Mutations C9A, K129C, D138C

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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