We narrowed to 1,041 results for: Psp

Showing: 931 - 960 of 1041 results

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SpVasa-K332N-GFP

Plasmid

#185592

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-K332N

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

K332N

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-S636K-GFP

Plasmid

#185589

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S636K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

S636K

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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mCm7RNA6A

Plasmid

#84367

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2 m7-gRNA6

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA5

Plasmid

#84366

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH3.2-GS3

Plasmid

#204763

Purpose

Expression of Cas9 and human H3.2

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H3.2 (HIST2H3PS2 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCPH2A.J-GS3

Plasmid

#204757

Purpose

Expression of Cas9 and human H2A.J

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AJ (H2AJ Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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L0I87-SPCHPromoter_GG0-1

Plasmid

#219707

Purpose

Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

Depositor

Jennifer Nemhauser

Insert

GG0-pSPCH-GG1

Use

Synthetic Biology

Mutation

WT promoter with added golden gate linkers

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHHYTK157

Plasmid

#200151

Purpose

Type 2 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pSP6_Kozak

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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EED-F97A

Plasmid

#131326

Purpose

gRNA to generate F97A cage-mutant EED

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG1B_p.P30S

Plasmid

#81291

Purpose

Gateway Donor vector containing REG1B ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG1B (REG1B Human)

Use

Gateway Cloning

Mutation

P30S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG1B_p.C64W

Plasmid

#81298

Purpose

Gateway Donor vector containing REG1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG1B (REG1B Human)

Use

Gateway Cloning

Mutation

C64W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG1B_p.A82T

Plasmid

#81276

Purpose

Gateway Donor vector containing REG1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG1B (REG1B Human)

Use

Gateway Cloning

Mutation

A82T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG1B_p.K157T

Plasmid

#81283

Purpose

Gateway Donor vector containing REG1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG1B (REG1B Human)

Use

Gateway Cloning

Mutation

K157T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pF10 Xfin (DM#125)

Plasmid

#15053

Depositor

Douglas Melton

Article

Ruiz i Altaba et al EMBO J. 1987 Oct . 6(10):3065-70.

Insert

Xfin fragment (znf208 Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pF5 Xfin (DM#126)

Plasmid

#15054

Depositor

Douglas Melton

Article

Ruiz i Altaba et al EMBO J. 1987 Oct . 6(10):3065-70.

Insert

Xfin fragment (znf208 Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pCPH2A.B-GS3

Plasmid

#204760

Purpose

Expression of Cas9 and human H2A.B

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AB1 (H2AFB1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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MiniSOG-SpAGS-GFP

Plasmid

#185581

Purpose

for CALI

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

miniSOG-SpAGS

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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5xtetO HoxC 3'

Plasmid

#131340

Purpose

gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

HoxC-downstream-gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA1_pNeurog2-Luciferase reporter

Plasmid

#64160

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for pNeurog2-Luciferase reporter

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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ZP59 23f17

Plasmid

#16883

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

23f17

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCPH2A.L-GS3

Plasmid

#204756

Purpose

Expression of Cas9 and human H2A.L

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AL3 (H2AC6 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa-C3

Plasmid

#185587

Purpose

to recruit Vasa-C3 to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-C3

Use

In vitro transcription (mrna synthesis)

Tags

mCherry and membrane targeting sequence from Neur…

Mutation

C3 mutant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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membrane-mCherry-SpVasa

Plasmid

#185586

Purpose

to recruit Vasa to membrane

Depositor

Mamiko Yajima

Article

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

mCherry, S-Tag and membrane targeting sequence fr…

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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