We narrowed to 1,005 results for: Psp

1. P63-sp64 Poly A

   Plasmid

   #59435

   Purpose

   synthetic poly A transcripts of VZV

   Depositor

   Randall Cohrs

   Article

   Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

   Insert

   VZV ORF 63
   

   Use

   Makes synthetic transcripts that contains poly a

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

2. P29-sp64 Poly A

   Plasmid

   #59434

   Purpose

   synthetic poly A transcripts of VZV

   Depositor

   Randall Cohrs

   Article

   Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

   Insert

   VZV ORF 29
   

   Use

   Makes synthetic transcripts that contains poly a

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Mouse c-myc (exons II and III)

   Plasmid

   #8448

   Depositor

   Frederic Mushinski

   Article

   Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

   Insert

   Mouse c-myc (exons II and III) (Myc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Exons II and III only

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

4. P21-sp64 Poly A

   Plasmid

   #59433

   Purpose

   synthetic poly A transcripts of VZV

   Depositor

   Randall Cohrs

   Article

   Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

   Insert

   VZV ORF 21
   

   Use

   Makes synthetic transcripts that contains poly a

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Rev CDK9 (D167N) (P#858)

   Plasmid

   #14635

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   Rev

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant.

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCJ1023

   Plasmid

   #228763

   Purpose

   Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABEmax-Puro V2

   Plasmid

   #226955

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHC025 (∆CBBp)

   Plasmid

   #226203

   Purpose

   Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCPH2A.X_K5Q-GS3

   Plasmid

   #204746

   Purpose

   Expression of Cas9 and human H2AX with the K5Q mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K5Q (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K5Q mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCPH2A.X_K5R-GS3

    Plasmid

    #204747

    Purpose

    Expression of Cas9 and human H2AX with the K5R mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5R (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K5R mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPH2A.X_K134L-GS3

    Plasmid

    #204750

    Purpose

    Expression of Cas9 and human H2AX with the K134L mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134L (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134L mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCPH2A.X_K134M-GS3

    Plasmid

    #204751

    Purpose

    Expression of Cas9 and human H2AX with the K134M mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134M (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134M mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCPH2A.X_K134A-GS3

    Plasmid

    #204752

    Purpose

    Expression of Cas9 and human H2AX with the K134A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134A mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDG462

    Plasmid

    #100903

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

19. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

20. RBM14_pLX307

    Plasmid

    #98367

    Purpose

    Lentiviral expression of RBM14

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    RBM14 (RBM14 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pXPE

    Plasmid

    #15284

    Depositor

    Ron Vale

    Article

    Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

    Insert

    ubiquitous kinesin heavy chain (KIF5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The insert is missing the 1st 180 bp of 5' u…

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

22. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

23. sgAAVS1(A)

    Plasmid

    #85570

    Purpose

    Expresses sgRNA targeting human AAVS1 locus

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    adeno-associated virus integration site 1 (AAVS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

24. sgAAVS1(B)

    Plasmid

    #85571

    Purpose

    Expresses sgRNA targeting human AAVS1 locus

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    adeno-associated virus integration site 1 (AAVS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

25. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

    Plasmid

    #91882

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

26. PX459v2-ILK-gRNA 1-exon 1

    Plasmid

    #163320

    Purpose

    SpCas9 ILK gRNA 1 (G*CGGAGAACGACCTCAACCAG), targeting exon 1 within the N-terminal ankyrin repeat domain-1.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 1_Exon1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

27. PX459v2-ILK-gRNA 2-exon 8

    Plasmid

    #163321

    Purpose

    SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ILK gRNA 2_Exon8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6F

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

28. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. INS-3'gRNA

    Plasmid

    #210468

    Purpose

    gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS (INS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS10-riboE-dddAI

    Plasmid

    #186561

    Purpose

    Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pSpac(hy)

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge